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Cadaverine: a common polyamine in zygotic embryos and somatic embryos of the species Capsicum chinense Jacq.

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Abstract

The behavior of endogenous polyamines was studied in somatic embryos and zygotic embryos of Habanero pepper (Capsicum chinense). In the first part of the work, the polyamine content was evaluated in both types of embryos (somatic and zygotic). As a result, in addition to the common polyamines (putrescine, spermidine and spermine), it was also possible to detect cadaverine, a polyamine rarely found in plants. In general, all the polyamines were found to be more abundant in somatic embryos than in zygotic embryos, with significantly higher contents of putrescine and cadaverine. Subsequently, the content of putrescine, spermidine, spermine and cadaverine, in their different forms (free, bound and conjugated) was determined in somatic embryos which were cultured in non-ventilated and ventilated containers. Detection of polyamines was carried out at 28 and 42 days of culture by the HPLC method. The ethylene content was monitored during the process in both culture conditions (ventilated and non-ventilated). As a result of the analysis, cadaverine was always found present, indicating that it is a common polyamine in the species. Ethylene was detected in containers without ventilation throughout the culture, except during replenishment of the culture medium (R1, R2 and R3). The behavior pattern of each polyamine, analyzed under different culture conditions (ventilated and non-ventilated) and at different moments of culture (28 and 42 days of culture), show that the polyamines are not only involved in morphogenic processes in plants; polyamines are also significantly affected by the surrounding environment. However, the most novel result, presented for the first time in this paper, is that cadaverine is found to be a common polyamine in C. chinense since it is present in both zygotic embryos and somatic embryos.

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Acknowledgments

This project was supported by the National Council of Science and Technology (CONACyT), Mexico.

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  1. Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43 # 130, Chuburná de Hidalgo, CP 97200, Mérida, Yucatán, Mexico

    Carlos Fernando Regla-Márquez, Adriana Canto-Flick, Susana Alejandra Avilés-Viñas, Raúl Enrique Valle-Gough, Jacobo Pérez-Pastrana, Francisco Javier García-Villalobos & Nancy Santana-Buzzy

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  1. Carlos Fernando Regla-Márquez
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  2. Adriana Canto-Flick
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Correspondence to Nancy Santana-Buzzy.

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Regla-Márquez, C.F., Canto-Flick, A., Avilés-Viñas, S.A. et al. Cadaverine: a common polyamine in zygotic embryos and somatic embryos of the species Capsicum chinense Jacq.. Plant Cell Tiss Organ Cult 124, 253–264 (2016). https://doi.org/10.1007/s11240-015-0889-x

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