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A comparison of genetic stability in tea [Camellia sinensis (L.) Kuntze] plantlets derived from callus with plantlets from long-term in vitro propagation

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Abstract

Morphological variation, genotypic stability and genetic distances among micropropagated tea [Camellia sinensis (L.) Kuntze] cv. ‘Kolkhida’ plantlets after 7 years of propagation in vitro and among plantlets regenerated from callus were compared. Fifteen individuals, randomly chosen amongst a set of 100 plantlets regenerated from callus, and ten individuals, randomly chosen from a set of 100 micropropagated plants were used in the study. Morphological descriptors showed higher variability among callus-derived regenerants than in micropropagated plants. Flow cytometry analysis showed no significant variability of nuclear DNA content among micropropagated plants, however changes in DNA indicated aneuploidy in three out of ten callus-derived regenerants. ISSR analysis showed lesser genetic distances among micropropagated plantlets and maternal cultivar ‘Kolkhida’ compared with callus derived plantlets. Yet, there is some degree of genetic instability after long-term micropropagation. Results confirmed the hypothesis of genetic instability of some species during long term in vitro conservation.

Key message

Tea vegetative explants plants were propagated in vitro for 7 years. A comparison of tea plantlets derived from callus with plantlets from long-term in vitro propagation revealed genetic variability and confirmed the hypothesis of genotype instability of tea plant.

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Abbreviations

MS :

Murashige and Skoog (1962)

DNA :

Deoxyribonucleic acid

RAPD :

Randomly amplified polymorphic DNA

ISSR :

Inter-simple sequence repeat

SSR :

Simple sequence repeat

RFLP :

Restriction fragment length polymorphism

2,4-D :

2,4-Dichlorophenoxy acetic acid

NAA :

Naphthalene acetic acid

BAP :

6-Benzylaminopurine

GA 3 :

Gibberelic acid

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Acknowledgements

This study was conducted under the financial support of Russian Science Foundation (Project #18-76-10001).

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Authors and Affiliations

  1. Federal State Budgetary Scientific Institution “Russian Research Institute of Floriculture and Subtropical Crops”, Yana Fabritsiusa 2/28, Sochi, Russia, 354002

    Lidiia Samarina, Maya Gvasaliya, Natalia Koninskaya, Ruslan Rakhmangulov, Alexander Efremov, Natalia Kiselyova & Alexey Ryndin

  2. Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Fruit Crops, Dresden, Germany

    Magda-Viola Hanke

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  1. Lidiia Samarina
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  2. Maya Gvasaliya
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  3. Natalia Koninskaya
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  4. Ruslan Rakhmangulov
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  5. Alexander Efremov
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  6. Natalia Kiselyova
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  8. Magda-Viola Hanke
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Contributions

L.S. performed the experiments, the interpretation of data and partly wrote the manuscript, M.G. performed the experiments on micropropagation, N.K. performed the experiments, R.R. performed the experiments, A.E. performed the experiments, N.K. performed statistical data processing, A.R.—scientific consultant, M.-V.H. contributed to the manuscript and critical revised the article.

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Correspondence to Lidiia Samarina.

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Communicated by Sergio J. Ochatt.

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Samarina, L., Gvasaliya, M., Koninskaya, N. et al. A comparison of genetic stability in tea [Camellia sinensis (L.) Kuntze] plantlets derived from callus with plantlets from long-term in vitro propagation. Plant Cell Tiss Organ Cult 138, 467–474 (2019). https://doi.org/10.1007/s11240-019-01642-2

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