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Isolation, characterization, and genomic analysis of vB_PaeS_TUMS_P81, a lytic bacteriophage against Pseudomonas aeruginosa

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Abstract

Pseudomonas aeruginosa is an opportunistic human pathogen that can lead to nosocomial infections which are in turn life threatening. The increase in antibiotic resistance, at an alarming rate, has resulted in a pressing need for alternative therapeutic approaches such as phage therapy, which hold promise according to several studies. This study featured the isolation and characterization of vB_PaeS_TUMS_P81, a new lytic Pseudomonas phage. The whole-genome sequencing indicated that it has a genome of 73,167 bp containing 93 predicted coding sequences. Genes involved in virulence or lysogeny pathway were nowhere to be found in the genome, so it is potentially safe when it comes to therapeutic applications. Genomic and phylogenetic analysis indicated that vB_PaeS_TUMS_P81 is a member of the genus Litunavirus, belonging to Schitoviridae family. The present study lays the groundwork for further research on treatment of P. aeruginosa infections.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

We thank Dr. M. Douraghi, from Tehran University of Medical Sciences, IKHC and Noor Reference Laboratory for providing bacterial strains; Dr. S. Sabouri Shahrbabak for her kind assistance; and Mr. S. Delara for his invaluable help writing the manuscript.

Funding

This work was supported by the National Institute for Medical Research Development (NIMAD), Grant no. 964206.

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Authors and Affiliations

  1. Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

    Haniyeh Kamyab, Narges Torkashvand, Ahmad Reza Shahverdi & Zargham Sepehrizadeh

  2. Department of Food and Drug Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

    Mohammad Reza Khoshayand

  3. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

    Mohammad Sharifzadeh

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  1. Haniyeh Kamyab
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Contributions

Haniyeh Kamyab: Data curation; Formal analysis; Investigation; Methodology; Writing -original draft; Software. Narges Torkashvand: Data curation; Formal analysis; Investigation; Methodology; Software. Ahmad Reza Shahverdi: Supervision, Project administration; Funding acquisition. Mohammad Reza Khoshayand: Supervision; Validation. Mohammad Sharifzadeh: Supervision; Validation. Zargham Sepehrizadeh: Conceptualization, Supervision, Project administration, Funding acquisition, Writing—review and editing.

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Correspondence to Zargham Sepehrizadeh.

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Kamyab, H., Torkashvand, N., Shahverdi, A.R. et al. Isolation, characterization, and genomic analysis of vB_PaeS_TUMS_P81, a lytic bacteriophage against Pseudomonas aeruginosa. Virus Genes 59, 132–141 (2023). https://doi.org/10.1007/s11262-022-01954-0

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