Abstract
Helicobacter pylori infection (H. pylori) is associated with chronic gastritis, peptic ulcers, and gastric cancer. The present study provides information on the protective effects of Limosilactobacillus reuteri strain 2892 (L. reuteri 2892) isolated from camel’s milk against H. pylori-induced gastritis in the stomach tissue of animal models. Animal assays revealed that L. reuteri 2892 pretreatment significantly downregulated the virulence factor cagA gene expression. It upregulated the expression level of tight junction molecules [zona occludens (ZO-1), claudin-4] and suppressed metalloproteinase (MMP)-2 and MMP-9 expressions. L. reuteri 2892 exhibited immunomodulatory effects on cytokine profile, as it reduced the serum concentrations of pro-inflammatory cytokines interleukin (IL)-6, IL-1β, and INF-γ and increased the anti-inflammatory cytokine, IL-10. In addition, L. reuteri 2892 showed anti-oxidative stress activity by regulating the levels of oxidative stress-associated markers [superoxide dismutase (SOD) and malondialdehyde (MDA)]. Our findings suggest that L. reuteri 2892 attenuates H. pylori-induced gastritis.
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Data availability
The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
Abbreviations
- HP:
-
Helicobacter pylori, H. pylori
- WHO:
-
World Health Organization
- L. acidophilus:
-
Lactobacillus acidophilus
- L. rhamnosus:
-
Lactobacillus rhamnosus
- L. plantarum:
-
Lactobacillus plantarum
- LAB:
-
Lactic acid bacteria
- L. reuteri:
-
Limosilactobacillus reuteri
- cagA:
-
cytotoxin associated antigen A
- vacA:
-
vacuolization cytotoxin
- FBS:
-
fetal bovine serum
- PBS:
-
phosphate-buffered saline
- MRS:
-
de Man, Rogosa, and Sharpe
- Pb:
-
Probiotics
- rDNA:
-
ribosomal DNA
- RT-qPCR:
-
real-time quantitative polymerase chain reaction
- ELISA:
-
enzyme-linked immunosorbent assay
- MDA:
-
malondialdehyde
- SOD:
-
superoxide dismutase
- MMP:
-
matrix metalloproteinases
- ZO-1:
-
zona occludens
- PMNs:
-
Polymorphonuclears
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Acknowledgments
The authors would like to thank the staff at Islamic Azad University of Shiraz, Islamic Azad University of Neyshabur, and the Neyshabur University of Medical Sciences for their sincere assistance and efforts to make this project happen
Funding
This work was supported by Islamic Azad University of Shiraz (Grant numbers [162449566]).
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FFN and AR performed the experiments, analyzed data, prepared the figures, and drafted the manuscript. MA analyzed pathological data. JS designed the project and experiments. AG and MM conceived, designed and supervised all aspects of the work, critically reviewed and edited the manuscript. All authors read and approved the final manuscript.
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The animal study was reviewed and approved by the Animal Ethics Committee of Islamic Azad University of Shiraz under code number IR.IAU.SHIRAZ.REC.1401.015.
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Forooghi Nia, F., Rahmati, A., Ariamanesh, M. et al. The Anti-Helicobacter pylori effects of Limosilactobacillus reuteri strain 2892 isolated from Camel milk in C57BL/6 mice. World J Microbiol Biotechnol 39, 119 (2023). https://doi.org/10.1007/s11274-023-03555-x
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DOI: https://doi.org/10.1007/s11274-023-03555-x