Abstract
With immunohistochemical and Western blot techniques, P2X1 receptors were detected in the whole mouse gastrointestinal tract and pancreatic islets of mouse and human. (1) δ Cells containing somatostatin (SOM) in the stomach corpus, small intestines, distal colon, pancreatic islets of both mouse and human express P2X1 receptors; (2) strong immunofluorescence of P2X1 receptors was detected in smooth muscle fibers and capillary networks of the villus core of mouse intestine; and (3) P2X1 receptor-immunoreactive neurons were also detected widely in both mouse myenteric and submucosal plexuses, all of which express SOM. The present data implies that ATP via P2X1 receptors is involved in SOM release from pancreatic δ cells, enteric neurons, and capillary networks in villi.
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Funding
This work was supported by the National Natural Science Foundation of P. R. China (81471260 to Z Xiang), by the Qingnian Science Foundation of Changzheng Hospital, Shanghai, People’s Republic of China (2016CZQN05 to R. Ji) and by the Qiannian Science Foundation of Second Military Medical University of P.L.A (2017QN15).
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Ruihua Ji declares that she has no conflict of interest.
Jiao Zhu declares that she has no conflict of interest.
Dan Wang declares that she has no conflict of interest.
Qian-Qian Sui declares that she has no conflict of interest.
Gillian E. Knight declares that she has no conflict of interest.
Geoffrey Burnstock declares that he has no conflict of interest.
Hongbin Yuan declares that she has no conflict of interest.
Zhenghua Xiang declares that she has no conflict of interest.
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All experimental procedures were approved by the Institutional Animal Care and Use Committee at Second Military Medical University and conformed to the UK Animals (Scientific Procedures) Act 1986 and associated guidelines on the ethical use of animals.
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Ji, R., Zhu, J., Wang, D. et al. Expression of P2X1 receptors in somatostatin-containing cells in mouse gastrointestinal tract and pancreatic islets of both mouse and human. Purinergic Signalling 14, 285–298 (2018). https://doi.org/10.1007/s11302-018-9615-6
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DOI: https://doi.org/10.1007/s11302-018-9615-6