Abstract
Background
Cord blood lipids are potential disease biomarkers. We aimed to determine if their concentrations were affected by delayed blood processing.
Method
Refrigerated cord blood from six healthy newborns was centrifuged every 12 h for 4 days. Plasma lipids were analysed by liquid chromatography/mass spectroscopy.
Results
Of 262 lipids identified, only eight varied significantly over time. These comprised three dihexosylceramides, two phosphatidylserines and two phosphatidylethanolamines whose relative concentrations increased and one sphingomyelin that decreased.
Conclusion
Delay in separation of plasma from refrigerated cord blood has minimal effect overall on the plasma lipidome.
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Acknowledgements
We thank and Ngaire Elwood and Linda Chilcott for assistance with cord blood collection. This research was supported by the Juvenile Diabetes Research Foundation (JDRF) Australia, the Australian Research Council Special Research Initiative in Type 1 Juvenile Diabetes, The Helmsley Charitable Trust, JDRF International, and the National Health and Medical Research Council (NHMRC) of Australia (Program Grants 1037321 and 1054618). LCH is the recipient of a NHMRC Senior Principal Research Fellowship (1080887). The work was made possible through Victorian State Government Operational Infrastructure Support and NHMRC Research Institute Infrastructure Support Scheme.
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The study was devised by JMW, MASP and LCH, who with EB-S arranged sample collection and storage. Lipid data were generated by KK and analysed by KK and NGB. JMW and LCH drafted the manuscript and all authors contributed to the final version.
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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
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Wentworth, J.M., Bediaga, N.G., Penno, M.A.S. et al. Minimal variation of the plasma lipidome after delayed processing of neonatal cord blood. Metabolomics 14, 130 (2018). https://doi.org/10.1007/s11306-018-1434-9
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DOI: https://doi.org/10.1007/s11306-018-1434-9