Abstract
Europium (Eu3+)-labeled antibody was used as a fluorescent label to develop a highly sensitive time-resolved fluoroimmunoassay (TRFIA) for determination of clothianidin residues in agricultural samples. Toward this goal, the Eu3+-labeled polyclonal antibody and goat anti-rabbit antibody were prepared for developing and evaluating direct competitive TRFIA (dc-TRFIA) and indirect competitive TRFIA (ic-TRFIA). Under optimal conditions, the half-maximal inhibition concentration (IC50) and the limit of detection (LOD, IC10) of clothianidin were 9.20 and 0.0909 μg/L for the dc-TRFIA and 2.07 and 0.0220 μg/L for the ic-TRFIA, respectively. The ic-TRFIA has no obvious cross-reactivity with the analogues of clothianidin except for dinotefuran. The average recoveries of clothianidin from spiked water, soil, cabbage, and rice samples were estimated to range from 74.1 to 115.9 %, with relative standard deviations of 3.3 to 11.7 %. The results of TRFIA for the blind samples were largely consistent with gas chromatography (R 2 = 0.9902). The optimized ic-TRFIA might become a sensitive and satisfactory analytical method for the quantitative monitoring of clothianidin residues in agricultural samples.
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References
Chen MF, Huang JW, Wong SS, Li GC (2005) Analysis of insecticide clothianidin and its metabolites in rice by liquid chromatography with a UV detector. J Food Drug Anal 13:279–283
Chen YJ, Wang Y, Li N, Gong ML (2009) Methodological study on the determination of residual pesticide in Brassica chinensis by HPLC. China Trop Med 9:361–362
Esteve-Turrillas FA, Parra J, Abad-Fuentes A, Agullo C, Abad-Somovilla A, Mercader JV (2010) Hapten synthesis, monoclonal antibody generation, and development of competitive immunoassays for the analysis of picoxystrobin in beer. Anal Chim Acta 682:93–103
Gui WJ, Jin MJ, Sun LF, Guo YR, Zhu GN (2009) Residues determination of carbofuran in vegetables based on sensitive time-resolved fluorescence immunoassay. Food Agric Immunol 20:49–56
Hagan AK, Zuchner T (2011) Lanthanide-based time-resolved luminescence immunoassays. Anal Bioanal Chem 400:2847–2864
Hou YY, Bian HZ, Zhao XX, Hu YF, Su T, Wang XH, Wan XC (2011) Determination of nicotinoid residues in complicated matrix vegetables by solid phase extraction and HPLC method. J Instrum Anal 30:58–63
Huang B, Zhao LL, Sun JM, Zhang J, Zhang Y, Zhang K, Jin J, Xie MH (2012) Establishment and validation of a time-resolved fluoroimmunoassay for chlorpromazine. Food Anal Methods 5:625–630
Kim BM, Park JS, Choi JH, El-Aty AM, Na TW, Shim JH (2012) Residual determination of clothianidin and its metabolites in three minor crops via tandem mass spectrometry. Food Chem 131:1546–1551
Kim MJ, Lee HS, Chung DH, Lee YT (2003) Synthesis of haptens of organophosphorus pesticide and development of enzyme-linked immunosorbent assays for parathion-methyl. Anal Chim Acta 493:47–62
Li L, Jiang GG, Liu CY, Liang HW, Sun DL, Li W (2012) Clothianidin dissipation in tomato and soil, and distribution in tomato peel and flesh. Food Control 25:265–269
Li M, Sheng EZ, Cong LJ, Wang MH (2013) Development of immunoassays for detecting clothianidin residue in agricultural products. J Agric Food Chem 61:3919–3623
Liu YH, Wang CM, Gui WJ, Bi JC, Jin MJ, Zhu GN (2009) Development of a sensitive competitive indirect ELISA for parathion residue in agricultural and environmental samples. Ecotox Environ Safe 72:1673–1679
Liu ZJ, Yu PM, Fang S, Fan JQ, Wang MH (2011) Development of an enzyme-linked immunosorbent assay for determination of pretilachlor in water and soil. Ecotox Environ Safe 74:1595–1599
Motohiro T, John YC (2005) Neonicotinoid insecticide toxicology: mechanisms of selective action. Annu Rev Pharmacol Toxicol 45:247–268
Sesay AM, Micheli L, Tervo P, Palleschi G, Virtanen V (2013) Development of a competitive immunoassay for the determination of cortisol in human saliva. Anal Biochem 434:308–314
Uchigashima M, Watanabe E, Ito S, Iwasa S, Miyake S (2012) Development of immunoassay based on monoclonal antibody reacted with the neonicotinoid insecticides clothianidin and dinotefuran. Sensors 12:15858–15872
Uneme H (2011) Chemistry of clothianidin and related compounds. J Agric Food Chem 59:2932–2937
Wengatz I, Stoutamire DW, Gee SJ, Hammock BD (1998) Development of an enzyme-linked immunosorbent assay for the detection of the pyrethroid insecticide fenpropathrin. J Agric Food Chem 46:2211–2221
Xu ZL, Dong JX, Yang JY, Wang H, Jiang YM, Lei HT, Shen YD, Sun YM (2012) Development of a sensitive time-resolved fluoroimmunoassay for organophosphorus pesticides in environmental water samples. Anal Methods 4:3484–3490
Zhang Z, Liu JF, Feng TT, Yao Y, Gao LH, Jiang GB (2013) Time-resolved fluoroimmunoassay as an advantageous analytical method for assessing the total concentration and environmental risk of fluoroquinolones in surface waters. Environ Sci Technol 47:454–462
Zhou YL, Xia XH, Xu Y, Ke W, Yang W, Li QG (2012) Application of europium(III) chelates-bonded silica nanoparticle in time-resolved immunofluorometric detection assay for human thyroid stimulating hormone. Anal Chim Acta 722:95–99
Acknowledgments
This work was supported by the National “863” High-Tech Research Program of China (no. 2011AA100806), the Special Fund for Agro-scientific Research in the Public Interest (no. 201203022), and the Project of Graduates’ Research Innovative Programs from Colleges and Universities of Jiangsu Province (no. CXZZ13-0305).
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Li, M., Sheng, E., Yuan, Y. et al. Sensitive time-resolved fluoroimmunoassay for quantitative determination of clothianidin in agricultural samples. Environ Sci Pollut Res 21, 5803–5809 (2014). https://doi.org/10.1007/s11356-014-2506-7
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DOI: https://doi.org/10.1007/s11356-014-2506-7