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Application of waste eggshell as a source of calcium in bacterial bio-cementation to enhance the engineering characteristics of sand

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Abstract

A technique to produce bio-cementation in sandy soil using the microbially induced calcium carbonate precipitation (MICP) process and calcium ions generated from eggshell is presented in this research. This research also focused on the application of S. pasteurii bacteria and L. fusiformis bacteria along with eggshell and calcium chloride cementing chemicals on the strength properties of sand. The experimental variables maintained in this research are bacteria type (S. pasteurii and L. fusiformis), cementing chemical type (eggshell and calcium chloride) and molarity of the cementing chemical (0.25, 0.50, 0.75 and 1.0). The engineering behaviour of bacteria treated sand was estimated by executing the unconfined compression test and permeability test in the laboratory. From the experimental findings, it is identified that the unconfined compressive strength of sand is enhanced and the value is in the range of 650 kPa. In addition to that, the permeability of sand is minimized in the order of two from 6.3 × E−3 to 3.2 × E−5 cm/s. The best improvement of Young’s modulus and calcium carbonate content estimated in this research are 28.9 MPa and 17.9% when the sand is treated with S. pasteurii along 0.50 molarity of eggshell cementing chemical. The experimental findings are validated with the help of microstructural studies of scanning electron microscopy (SEM) and energy-dispersive X-ray analysis (EDX). This research showed that bio-cementation technology in the form of S. pasteurii and eggshell can be effectively adopted to enhance the engineering characteristics of sand.

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References

  • Al Qabany AA, Soga K (2013) Effect of chemical treatment used in MICP on engineering properties of cemented soils. Geotechnique 63(4):331–339

    Article  Google Scholar 

  • Al Qabany AA, Soga K, Santamarina C (2012) Factors affecting efficiency of microbially induced calcite precipitation. J Geotech Geoenviron Eng 138(8):992–1001

    Article  Google Scholar 

  • Casey B, Germaine J, Abdulhadi N, Kontopoulos N, Jones C (2016) Undrained Young’s modulus of fine-grained soils. J Geotech Geoenviron Eng 142(2):04015070

    Article  Google Scholar 

  • Choi SG, Wu S, Chu J (2016) Biocementation for sand using an eggshell as calcium source. J Geotech Geoenviron Eng 142(10):06016010

    Article  Google Scholar 

  • Gomez MG, Graddy CMR, DeJong JT, Nelson DC, Tsesarsky M (2018) Stimulation of native microorganisms for biocementation in samples recovered from field-scale treatment depths. J Geotech Geoenviron Eng 144(1):040170981–0401709813

    Article  Google Scholar 

  • Gowthaman S, Mitsuyama K, Nakashima K, Komatsu M, Kawasaki S (2019a) Biogeotechnical approach for slope soil stabilization using locally isolated bacteria and inexpensive low-grade chemicals: a feasibility study on Hokkaido expressway soil. Japan’, Soils and Foundations 59:484–499

    Article  Google Scholar 

  • Gowthaman S, Iki T, Nakashima K, Ebina K, Kawasaki S (2019b)’ Feasibility study for slope soil stabilization by microbial induced carbonate precipitation (MICP) using indigenous bacteria isolated from cold subarctic region’. SN Appl Sci. https://doi.org/10.1007/s42452-019-1508-y

  • Gowthaman S, Nakashima K, Kawasaki S (2021) Effect of wetting and drying cycles on the durability of bio-cemented soil of expressway slope. Int J Environ Sci Technol 1–14

  • Kim D, Park K (2013) An environmentally friendly soil improvement technology with microorganism. International Journal of Railway 6(3):90–94

    Article  Google Scholar 

  • Kulanthaivel P, Soundara B, Das A (2020) Performance study on stabilization of fine grained clayey soils using calcium source producing microbes. KSCE Journal of Civil Engineering 24(9):2631–2642

    Article  Google Scholar 

  • Li D, Tian K, Zhang H, Wu Y, Nie K, Zhang S (2018) Experimental investigation of solidifying desert aeolian sand using microbially induced calcite precipitation. Constr Build Mater 172(5):251–262

    Google Scholar 

  • Lin H, Suleiman MT, Brown DG, Kavazanjian E Jr (2016) Mechanical behavior of sands treated by microbially induced carbonate precipitation. J Geotech Geoenviron Eng 142(2):04015066

    Article  Google Scholar 

  • Lv C, Zhu C, Tang CS, Cheng Q, Yin LY, Shi B (2020) ‘Effect of fiber reinforcement on the mechanical behavior of bio-cemented sand’. Geosynthetics Int 1–11

  • Omoregie AI, Ngu LH, Ong DEL, Nissom PM (2018) Low-cost cultivation of Sporosarcina pasteurii strain in food-grade yeast extract medium for microbially induced carbonate precipitation (MICP) application. Biocatal Agric Biotechnol. https://doi.org/10.1016/j.bcab.2018.11.030

    Article  Google Scholar 

  • Porter H, Dhami NK, Mukherjee A (2017) Synergistic chemical and microbial cementation for stabilization of aggregates. Cement Concr Compos 83:160–170

    Article  CAS  Google Scholar 

  • Selvakumar S, Kulanthaivel P, Soundara B (2021) Influence of nano-silica and sodium silicate on the strength characteristics of clay soil’. Nanotechnol Environ Eng 6(3):1–10

    Article  Google Scholar 

  • Sharaky AM, Mohamed NS, Elmashad ME, Shredah NM (2018) Application of microbial biocementation to improve the physic-mechanical properties of sandy soil. Constr Build Mater 190:861–869

    Article  CAS  Google Scholar 

  • Sharma M, Satyam N, Reddy KR (2021a) Hybrid bacteria mediated cemented sand: Microcharacterization, permeability, strength, shear wave velocity, stress-strain and durability. Int J Damage Mech 30(4):618–645

    Article  CAS  Google Scholar 

  • Sharma M, Satyam N, Reddy KR (2021b) State of the art review of emerging and biogeotechnical methods for liquefaction mitigation in sands. Journal of Hazardous, Toxic and Radioactive Waste 25(1):031200021–0312000222

    Google Scholar 

  • Sharma M, Satyam N, Reddy KR (2019) ‘Investigation of various gram-positive bacteria for MICP in Narmada Sand, India’. Int J Geotech Eng 1–15

  • Sidik WS, Canakci H, Kilic IH, Celik F (2014) Applicability of biocementation for organic soil and its effect on permeability. Geomech Eng 7(6):649–663

    Article  Google Scholar 

  • Soon NW, Lee LM, Khun TC, Ling HS (2013) Improvements in engineering properties of soils through microbial-induced calcite precipitation. KSCE J Civ Eng 17(4):718–728

    Article  Google Scholar 

  • Stróżyk J, Tankiewicz M (2016) The elastic undrained modulus Eu50 for stiff consolidated clays related to the concept of stress history and normalized soil properties. Studia Geotech Mech 38(3):67–72

    Article  Google Scholar 

  • Yasuhara H, Neupane D, Hayashi K, Okamura M (2012) Experiments and predictions of physical properties of sand cemented by enzymatically-induced carbonate precipitation. Soils and Foundations. 52(3):539–549

    Article  Google Scholar 

  • Yuan H, Liu K, Zhang C, Zhao Z (2021) Mechanical properties of Na-montmorillonite-modified EICP-treated silty sand. Environ Sci Pollut Res. https://doi.org/10.1007/s11356-021-16442-5

    Article  Google Scholar 

  • Zamani A, Montoya BM (2017) Shearing and hydraulic behavior of MICP treated silty sand. In: Brandon TL, Valentine RJ (eds) Geotechnical Frontiers 2017: Seismic Performance and Liquefaction, Geotechnical Special Publication 281. ASCE, Reston, VA, pp 290–299

    Chapter  Google Scholar 

  • Zhao Q, Li L, Li C, Li M, Amini F, Zhang H (2014) ‘Factors affecting improvement of engineering properties of MICP-treated soil catalyzed by bacteria and urease’, Journal of Materials in Civil Engineering, vol.26, no.12, pp.04014094–1–11

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PK conceptualized the presented idea, derived the methodology, designed and performed the experiments and wrote the original draft. BS developed the theory, supervised the findings and supported in writing review and editing. SS article drafting and contributed to the final version of the manuscript and supported in writing review. AD contributed to final version and supported in writing review and editing the manuscript.

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Correspondence to Subburaj Selvakumar.

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Kulanthaivel, P., Soundara, B., Selvakumar, S. et al. Application of waste eggshell as a source of calcium in bacterial bio-cementation to enhance the engineering characteristics of sand. Environ Sci Pollut Res 29, 66450–66461 (2022). https://doi.org/10.1007/s11356-022-20484-8

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