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Abundance and community structure of ammonia oxidizing bacteria and archaea in a Sweden boreal forest soil under 19-year fertilization and 12-year warming

  • SOILS, SEC 5 • SOIL AND LANDSCAPE ECOLOGY • RESEARCH ARTICLE
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Abstract

Purpose

Boreal forests are considered to be more sensitive to global climate change compared with other terrestrial ecosystems, but the long-term impact of climate change and forest management on soil microbial functional diversity is not well understood. Ammonia-oxidizing bacteria (AOB) and archaea (AOA) are the most important players in nitrogen (N) cycling-associated processes in terrestrial ecosystems. This study investigated the separate and combined impacts of long-term soil warming and fertilization on soil AOB and AOA community structures and abundances in a Norway spruce stand in northern Sweden.

Materials and methods

The soil-warming experiment was established in the buffer zones of two irrigated plots (I) and complete nutrient solution plots (IL) since 1995. The warming treatment started in April each year by maintaining soil temperature on warmed plots at 5°C above the temperature in unwarmed plots using heating cables. In August 2006, soil samples were collected from eight subplots for molecular analysis. The abundance of bacterial and archaeal amoA genes was determined by quantitative polymerase chain reaction. Similarly, total bacterial and archaeal population sizes have also been determined. The diversity of AOB and AOA was assessed by constructing amoA gene clone libraries, and different genotypes were screened with restriction fragment length polymorphism.

Results and discussion

Results showed that fertilization did not significantly affect the abundance of the bacterial amoA gene under either warming or non-warming conditions; however, warming decreased the abundance under fertilization treatments. No significant effects of fertilization and soil warming were observed on the number of thaumarchaeal amoA gene copies across all treatments. In this study, amoA gene abundance of AOB was significantly higher than that of AOA across all treatments. The community structure of both AOB and AOA was strongly influenced by fertilization. For bacterial amoA genes, Nitrosospira cluster 2 was present across all treatments, but the only genotype was observed in the fertilization treatments while, for thaumarchaeal amoA genes, the relative abundance of soil cluster 5 increased in fertilization treatments. By comparison, soil-warming effects on AOB and AOA community structure were not significant. Canonical correspondence analysis showed a positive correlation between fertilization and both dominant genotypes of AOB and AOA.

Conclusions

These results indicated that the abundance of AOA and AOB was not affected by fertilization or warming alone, but the interaction of fertilization and warming reduced the abundance of AOB. The community composition of ammonia-oxidizers was more affected by the nutrient-optimized fertilization than the soil warming.

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Acknowledgments

This research was jointly supported by Australian Research Council (FT0990547; DP0664154; DP1092470), Chinese Academy of Sciences (KZCX2-YW-JC401), Natural Science Foundation of China (41020114001, 41025004), and the Swedish University of Agricultural Sciences. Initial establishment and maintenance of the long-term soil-warming experiment was supported by the former Swedish Council of Forestry and Agricultural Research (SJFR) and later by the Swedish Research Council for Environment, Agricultural Sciences, and Spatial Planning (FORMAS).

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Authors and Affiliations

  1. State Key Laboratory of Urban and Regional Ecology, Research Centre for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, People’s Republic of China

    Xien Long & Jizheng He

  2. Graduate University, Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Xien Long

  3. Environmental Futures Centre and School of Biomolecular and Physical Sciences, Griffith University, Nathan, Brisbane, QLD, 4111, Australia

    Xien Long & Zhihong Xu

  4. Environmental Futures Centre and Griffith School of Environment, Griffith University, Nathan, Brisbane, QLD, 4111, Australia

    Chengrong Chen

  5. Southern Swedish Forest Research Centre, Swedish University of Agricultural Sciences, P.O. Box 49, SE-230 53, Alnarp, Sweden

    Sune Linder

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  1. Xien Long
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Correspondence to Chengrong Chen or Jizheng He.

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Long, X., Chen, C., Xu, Z. et al. Abundance and community structure of ammonia oxidizing bacteria and archaea in a Sweden boreal forest soil under 19-year fertilization and 12-year warming. J Soils Sediments 12, 1124–1133 (2012). https://doi.org/10.1007/s11368-012-0532-y

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