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A monoclonal antibody-based immunoassay for the determination of oxyresveratrol from Artocarpus lacucha Buch.-Ham.

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Abstract

Oxyresveratrol is a major active compound in the heartwood of Artocarpus lacucha. It plays an important role in anti-tyrosinase, antioxidant, anti-inflammatory, antiviral and neuroprotective properties. There are many A. lacucha commercial products available on the market for skin whitening and anti-aging effects. To evaluate the quality of raw material from the plant, a monoclonal antibody (MAb) against oxyresveratrol was generated in this study. The immunogen was prepared by the Mannich reaction for the conjugation of oxyresveratrol and cationized bovine serum albumin (cBSA). The conjugation of oxyresveratrol–cBSA at a ratio of 1:50 was used for the immunization. The novel MAb (E4) was specific to oxyresveratrol and resveratrol. An indirect competitive enzyme-linked immunosorbent assay (ELISA) using the MAb (E4) was developed for the determination of oxyresveratrol. The linear range for the measurement of oxyresveratrol was 63–500 ng/mL and the precision (% relative standard deviation) was found to be <10% with the percentages of recovery from 95.93−103.55%. According to the validation analysis, the established ELISA can be applied for the determination of oxyresvertrol in the heartwood of A. lacucha and samples of the traditional drug Puag-Haad. With reliability and high sensitivity, this assay can provide an alternative approach for the quantitative analysis of oxyresveratrol in A. lacucha samples.

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Acknowledgements

This research work was supported by a grant from the National Research Council of Thailand (GRB_APS_14_58_33_02), with additional funding from Khon Kaen University, Thailand.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand

    Tharita Kitisripanya, Chadathorn Inyai, Supaluk Krittanai & Waraporn Putalun

  2. Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen, 40002, Thailand

    Tharita Kitisripanya, Chadathorn Inyai, Supaluk Krittanai & Waraporn Putalun

  3. Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand

    Kittisak Likhitwitayawuid & Boonchoo Sritularak

  4. Laboratory of Medicinal Chemistry, Program in Chemical Biology, Chulabhorn Research Institute, Chulabhorn Graduate Institute, 54 Kampaeng Phet 6 Road, Bangkok, Thailand

    Poonsakdi Ploypradith

  5. Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, 812-8582, Japan

    Hiroyuki Tanaka & Satoshi Morimoto

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  1. Tharita Kitisripanya
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  2. Chadathorn Inyai
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  3. Supaluk Krittanai
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  4. Kittisak Likhitwitayawuid
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  5. Boonchoo Sritularak
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  6. Poonsakdi Ploypradith
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  7. Hiroyuki Tanaka
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  8. Satoshi Morimoto
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  9. Waraporn Putalun
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Correspondence to Waraporn Putalun.

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Kitisripanya, T., Inyai, C., Krittanai, S. et al. A monoclonal antibody-based immunoassay for the determination of oxyresveratrol from Artocarpus lacucha Buch.-Ham.. J Nat Med 71, 523–530 (2017). https://doi.org/10.1007/s11418-017-1083-8

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  • DOI: https://doi.org/10.1007/s11418-017-1083-8

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