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Heterosis and combining ability evaluation for growth traits of blunt snout bream (Megalobrama amblycephala) when crossbreeding three strains

  • Article
  • Marine & Freshwater Biology
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Chinese Science Bulletin

Abstract

The blunt snout bream (Megalobrama amblycephala) is a major aquaculture species in the Chinese freshwater polyculture system. In this study, complete diallel crossing of three strains (i.e., Liangzi [LZ], Poyang [PY] and Yuni [YN]) was used to evaluate the combining ability and heterosis effect of intraspecific crossbreeding on the growth performance (i.e., body weight, total length, body length, body height, and Fulton’s coefficient of condition). The offspring produced from the three strains were reared in a communal pond for 20 months, and nine microsatellites were used to assess their pedigree. Of the 749 offspring, 708 (94.53 %) could be assigned directly to a single parental pair. Significant differences were observed in body weight (BW) among the different combinations, indicating that the combining ability of BW from each strain was significantly different. The general combining ability (GCA) of BW from the sire was much higher than that from the dam. The GCA for the dam and sire of the LZ and YN were the largest and the smallest, respectively. The special combining ability of YN♀ × PY♂ was the largest, and YN♀ × PY♂ showed significantly positive heterosis effects on all F1 growth traits, except for Fulton’s condition coefficient (K) (P < 0.05). In addition, significant positive linear correlations were found between the mean BW of corresponding progeny and the H E of the parental generation (y = 600.7x − 29.472, r = 0.8651, P = 0.0026), H O (y = 1206.9x − 518.14, r = 0.7436, P = 0.0216), and the polymorphism information content (y = 1021.3x − 331.31, r = 0.8245, P = 0.0063) as determined by a correlative test. The findings obtained from the study will be important for future considerations of M. amblycephala stock improvement programs.

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Acknowledgments

This work was supported by the Modern Agriculture Industry Technology System Construction Projects of China titled as—Staple Freshwater Fishes Industry Technology System (CARS-46-05), National Natural Science Foundation of China (31201988), Projects of Hubei Cooperation and Exchanges (2013BHE006), Wuhan Engineering Research Center of Breeding for Wuchang Bream, National Ministry of Science and Technology Support Program (2012BAD26B00), State Key Laboratory of Freshwater Ecology and Biotechnology of China (2012FB06), Excellent Youth Foundation of Hubei Scientific Committee (2013CFA032), and Fundamental Research Funds for the Central Universities (2013PY066).

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Authors and Affiliations

  1. College of Fisheries, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, China

    Wei Luo, Cong Zeng, Shaokui Yi, Weimin Wang & Zexia Gao

  2. Collaborative Innovation Center for Healthy Freshwater Aquaculture of Hubei Province, Wuhan, 430070, China

    Zexia Gao

  3. Nofima, PO Box 5010, 1432, Ås, Norway

    Nicholas Robinson

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  1. Wei Luo
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  2. Cong Zeng
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  3. Shaokui Yi
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  5. Weimin Wang
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Correspondence to Zexia Gao.

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Luo, W., Zeng, C., Yi, S. et al. Heterosis and combining ability evaluation for growth traits of blunt snout bream (Megalobrama amblycephala) when crossbreeding three strains. Chin. Sci. Bull. 59, 857–864 (2014). https://doi.org/10.1007/s11434-014-0115-y

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