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Cryoconservation and regeneration of axillary shoot meristems of Indigofera tinctoria (L.) by encapsulation–dehydration technique

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Abstract

In vitro axillary shoot proliferation was achieved from single-node explants of Indigofera tinctoria on a well-defined medium, Murashige and Skoog (MS) medium supplemented with 1.0 mg l−1 N 6-benzyl adenine (BA) and 0.1 mg l−1 indole-3-acetic acid. Axillary shoot meristems from cultures derived from up to three subcultures were used in the encapsulation–dehydration technique. Preconditioned, calcium alginate-encapsulated, and precultured axillary shoot meristems were subjected to different lengths of desiccation in a laminar flow cabinet. Maximum survival and regeneration rates of 56.7% and 62.2%, respectively, were obtained in half-strength (half the macro- and micronutrients and full-strength vitamins) MS medium supplemented with 0.5 mg l−1 gibberellic acid and 0.2 mg l−1 BA after 4 h of desiccation, during which the moisture content was reduced to 16.0%. According to the analysis of six random amplified polymorphic DNA markers, plantlets derived from cultures initiated with cryopreserved plant material were genetically identical to those derived from nonfrozen (control) tissues.

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Acknowledgments

The authors thank Dr. William Decruse, Scientist, Tropical Botanical Garden and Research Institute, Palode, Trivandrum, Kerala, India for his valuable advice in the in vitro conservation part of the study.

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  1. Department of Plantation Crops and Spices, College of Agriculture, Kerala Agricultural University, Trivandrum, Kerala, India

    Deepa S. Nair & B. R. Reghunath

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  1. Deepa S. Nair
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  2. B. R. Reghunath
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Correspondence to Deepa S. Nair.

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Editor: F. Englemann

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Nair, D.S., Reghunath, B.R. Cryoconservation and regeneration of axillary shoot meristems of Indigofera tinctoria (L.) by encapsulation–dehydration technique. In Vitro Cell.Dev.Biol.-Plant 45, 565–573 (2009). https://doi.org/10.1007/s11627-009-9244-4

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