Abstract
Cryopreservation is a valuable technique for the long-term conservation of plant germplasm and complementary to traditional seed storage methods. However, critical factors such as seed moisture content should be optimized before using this technique as a safe strategy for storing seeds such as those of Nicotiana spp. This study aimed to determine the effect of desiccation on physiological and biochemical indicators associated with germination and vigor in cryopreserved seeds of Nicotiana tabacum cv. Sancti Spíritus 96 (SS-96). The germination and vigor of seeds with a range of moisture content were assessed using electrolyte leakage and accelerated aging tests. In addition, these physiological indicators were related to the oxidative state of the seeds, in terms of the rate of O2 ·− generation and the H2O2 content, and the activity of enzymatic antioxidants superoxide dismutase and catalase. The cryopreserved seeds of N. tabacum SS-96 with a moisture content of 2.1% exhibited higher vigor probably due to the retention of membrane integrity, reflected by lower levels of lipid peroxidation and electrolyte leakage associated with the absence of oxidative stress. The results suggest 2.1% as the optimal moisture content for the storage of seeds of this cultivar, both at cryogenic temperatures and at 5°C.
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Acknowledgements
We thank Magister Gilberto Torrecilla Guerra and Magister Otilio Ruiz Padrón for advising and editing at initial steps of the work and the manuscript. We thank also the participation of Dr. Sershen at the end to help edit the manuscript for language and improved aspects related to data presentation, interpretation, and discussion. This study was part of JLPR’s Magister Thesis from University of Ciego de Avila (Cuba).
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This research was funded by the Cuban Ministry for Agriculture.
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Magister Juan Luis Pérez-Rodríguez (JLPR) was heavily involved in sourcing funding to support the project. Moreover, JLPR was involved in the design of all experiments, acquisition, analysis, and interpretation (statistical assessment) for the study. JLPR was also the primary person responsible for the initial drafting of the work (including the writing of manuscript and construction of figures). JLPR was also a major contributor providing detailed comments, additions, and major text modifications for drafts as the manuscript developed. This study was part of JLPR’s Magister Thesis from the University of Ciego de Avila (Cuba). Magister René Carlos Rodríguez Escriba (RCRE) and Magister Gustavo Lorente González (GLG) were heavily involved in the planning, design, analysis, and interpretation of the accelerated aging test experiment. Moreover, RCRE and GLG contributed to the determination of generation rate of O2 ·−, H2O2 content and enzymatic activities of superoxide dismutase (SOD) and catalase (CAT) of Nicotiana tabacum L. cv. Sancti Spíritus 96 seeds collected. RCRE and GLG were also involved in the interpretation of data and contributed for the initial drafting of the manuscript. Dr. Justo González-Olmedo (JGO) was involved in the design of the all experiments and the acquisition of data and data management to align with the original concept of the work. Dr. JGO was also involved in the writing of manuscript. Dr. Marcos Martinez-Montero (MMM) was heavily involved in sourcing funding to support the project and the conception and design of all experiments. MMM was also heavily involved in the acquisition of the data sets and the interpretation of data and its presentation in the manuscript. MMM was also a major contributor providing detailed comments, additions, and text modifications for different drafts of the manuscript. Finally, all authors approve the submission of this manuscript to be published and agree to be accountable for all aspects of the work.
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Pérez-Rodríguez, J.L., Rodríguez Escriba, R.C., Lorente González, G.Y. et al. Effect of desiccation on physiological and biochemical indicators associated with the germination and vigor of cryopreserved seeds of Nicotiana tabacum L. cv. Sancti Spíritus 96. In Vitro Cell.Dev.Biol.-Plant 53, 440–448 (2017). https://doi.org/10.1007/s11627-017-9857-y
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DOI: https://doi.org/10.1007/s11627-017-9857-y