Abstract
The aim of this study was to establish a system of in vitro germination and propagation of Guazuma ulmifolia Lam. microcuttings (Malvaceae). Seeds were inoculated into test tubes containing different concentrations (half-strength (½) and full-strength) of Murashige and Skoog (MS) basal salts and sucrose (0, 10, 20, 40, and 80 g L−1). For the induction of multiple shoots, microcuttings were cultured on MS medium supplemented with 0.5, 1.0, and 2.0 mg L−1 of 6-benzyladenine (BA), kinetin (KIN), or thidiazuron (TDZ), or containing no plant growth regulator (control). Nodal segments were also cultured in vessels with two different sealing systems, for example, sealed with a porous membrane (1 M) (21 μL L−1 s−1 of CO2) or with no membrane (0 M) (14 μL L−1 s−1 of CO2). Treatments with full-strength MS salts supplemented with 20% sucrose and ½ MS with 10% sucrose showed 100% germination, whereas there was no germination in the culture media with the highest sucrose concentration (80 g L−1), regardless of the MS salt concentration. The presence of cytokinins in the culture medium reduced shoot length and number of leaves and roots in the microcuttings. Plants cultured in vessels sealed with a porous membrane showed greater total and shoot lengths and greater leaf area. Anatomical analyses also showed that the mesophyll and leaf blade were thicker in plants cultured under higher gas exchange (1 M), a condition that also favored ex vitro plant acclimatization. These results provide relevant information for the establishment of G. ulmifolia micropropagation systems.
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Funding
This work was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brazil (CAPES)—Finance Code 001; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Grant 311282/2021–0); and Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) (Grant APQ-02581–21).
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MJS established in vitro cultures and wrote the paper; LLLD and LASS assisted with histological and morphometrical evaluations; GZS and EAF performed statistical analyses and revised the paper; DSB and WCO wrote the paper; and APCN and DIR designed the research and wrote the paper.
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de Jesus Santana, M., Barbosa-Júnior, S.M., Dias, L.L.L. et al. A novel in vitro propagation system for West Indian elm [Guazuma ulmifolia Lam. (Malvaceae)]: a valuable medicinal woody species. In Vitro Cell.Dev.Biol.-Plant 58, 865–875 (2022). https://doi.org/10.1007/s11627-022-10275-8
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DOI: https://doi.org/10.1007/s11627-022-10275-8