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A strategy for detecting absorbed bioactive compounds for quality control in the water extract of rhubarb by ultra performance liquid chromatography with photodiode array detector

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Abstract

Objective

To detect absorbed bioactive compounds of the water extract whose pharmacodynamic effect was craniocerebral protection for quality control assessment.

Methods

Anthraquinones in water extract of rhubarb (WER), in cerebrospinal fluid (CSF) of patients with traumatic brain injury (TBI) and in ipsilateral cortex of TBI rats following oral WER were respectively explored by ultra performance liquid chromatography with photodiode array detector (UPLC-PDA) method developed in the present study. The effects of anthraquinones absorbed into injured cortex on superoxidase dismutase (SOD) activity in TBI rats were detected. The antioxidative anthraquinones absorbed into target organ were evaluated for quality control of WER.

Results

Anthraquinones in WER were aloe-emodin, rhein, emodin, chrysophanol, and physcion. Only the last anthraquinone was found in CSF and in ipsilateral cortex under this chromatographic condition. Physcion increased SOD activity in TBI rats significantly.

Conclusions

Physcion was the main active compound of rhubarb against craniocerebral injury via antioxidant pathway. According to our strategy, the exploration of physcion suggested the possibility of a novel quality control of WER in treating TBI injury.

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Author information

Authors and Affiliations

  1. Institute of Integrated Traditional Chinese and Western Medicine, and the National Key Clinical Specialist Vocational School of Traditional Chinese Medicine Encephalopathy, Xiangya Hospital, Central South University, Changsha, 410008, China

    Yang Wang  (王 杨), Xi Huang  (黄 熙), Qing-hua Liang  (梁清华), Rong Fan  (范 荣), Feng Qin  (秦 锋), Kui-po Yan  (闫奎坡), Wei Liu  (刘 维), Jie-kun Luo  (罗杰坤) & Yun-hui Li  (李云辉)

  2. Department of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China

    Yang Wang  (王 杨), Xi Huang  (黄 熙), Qing-hua Liang  (梁清华), Rong Fan  (范 荣), Kui-po Yan  (闫奎坡), Wei Liu  (刘 维), Jie-kun Luo  (罗杰坤), Yun-hui Li  (李云辉) & Xi-lian Mao  (毛喜莲)

  3. Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, 410008, China

    Yong Guo  (呙 勇)

  4. Institute of Clinical Pharmacology, Central South University, Changsha, 410008, China

    Zhao-qian Liu  (刘昭前) & Hong-hao Zhou  (周宏灏)

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  1. Yang Wang  (王 杨)
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  2. Xi Huang  (黄 熙)
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  11. Xi-lian Mao  (毛喜莲)
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  13. Hong-hao Zhou  (周宏灏)
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Corresponding author

Correspondence to Xi Huang  (黄 熙).

Additional information

Supported by the Fund for Key Laboratory of Traditional Chinese Medicine Gan of State Administration of Traditional Chinese Medicine, the Major Research Plan of the National Natural Science Foundation of China (No. 90409010), and was partly supported by the Huge Project to Boost Chinese Drug Development (No. 2009ZX09304-003)

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Wang, Y., Huang, X., Liang, Qh. et al. A strategy for detecting absorbed bioactive compounds for quality control in the water extract of rhubarb by ultra performance liquid chromatography with photodiode array detector. Chin. J. Integr. Med. 18, 690–698 (2012). https://doi.org/10.1007/s11655-012-1053-7

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  • DOI: https://doi.org/10.1007/s11655-012-1053-7

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