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Structural characterization and antioxidant activity of a novel high-molecular-weight polysaccharide from Ziziphus Jujuba cv. Muzao

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Abstract

A novel high-molecular-weight polysaccharide (PZMP2-1) was extracted and purified from Ziziphus Jujuba cv. Muzao. Chemical and instrumental studies revealed that PZMP2-1 had an average molecular weight (Mw) of 141 kDa and consisted of rhamnose, arabinose, galactose, and galacturonic acid with the molar ratio of 0.84:5.88:0.31:0.12. Structural features of PZMP2-1 were investigated via FT-IR, XRD, SEM, AFM, NMR spectroscopy and GC–MS analysis. Methylation and NMR analysis indicated that PZMP2-1 primarily contained of → 5)-a-L-Araf-(1 → , and → 3,5)-a-L-Araf-(1 → residue in the backbone. The branched chain was connected to the main chain through the O-3 atom of arabinose, and terminated with Araf. The results of bioactivity experiments showed that PZMP2-1 had antioxidant in vitro, especially on scavenging DPPH and hydroxyl radicals. Overall, these results suggested that jujube polysaccharides could be developed as a potent antioxidant agent for use in functional food and dietary supplements.

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Acknowledgements

We thank the Natural Science Foundation of Henan Province (212300410297), Basic Research Plan of Higher Education School Key Scientific Research Project of Henan Province (21A550014), Doctoral Research Foundation of Zhengzhou University of Light Industry (2020BSJJ015), and Science and Technology Research Project of Higher Education in Hebei Province (QN2020233).

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  1. College of Food and Bioengineering, Henan Key Laboratory of Cold Chain Food Quality and Safety Control, Zhengzhou University of Light Industry, Zhengzhou, 450001, People’s Republic of China

    Xiaolong Ji, Jianhang Guo, Deqi Ding, Jie Gao, Lirui Hao & Yanqi Liu

  2. Basic Medical College, Hebei University of Chinese Medicine, Hebei Higher Education Institute Applied Technology Research Center on TCM Formula Preparation, Hebei TCM Formula Preparation Technology Innovation Center, Shijiazhuang, 050200, People’s Republic of China

    Xudan Guo

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  1. Xiaolong Ji
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Ji, X., Guo, J., Ding, D. et al. Structural characterization and antioxidant activity of a novel high-molecular-weight polysaccharide from Ziziphus Jujuba cv. Muzao. Food Measure 16, 2191–2200 (2022). https://doi.org/10.1007/s11694-022-01288-3

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