Abstract
Callus cultures of Tabernaemontana persicariaefolia, (Apocynaceae), an endangered species endemic to the Mascarene Islands, were established from leaf explants on MS medium containing either 5 mg·l−1 2,4-D and 0.5 mg·l−1 BA or 5 mg·l−1 2,4-D, 0.5 mg·l−1 BA and 200 mg·l−1 DFMO. Histological studies showed regenerating nodules resembling globular embryos in calli after 4 weeks on the DFMO medium. Green shoot formation was achieved by sequential subculture of the induced calli on media with gradually decreasing 2,4-D concentrations (5→1→0 mg·l−1). Regeneration was greatly stimulated in the presence of DFMO. The first emergence of shoots occured 3 weeks earlier than in untreated callus cultures.
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Abbreviations
- BA:
-
6-benzyladenine
- 2,4-D:
-
2,4-Dichlorophenoxyacetic acid
- DFMO:
-
∝-difluoromethylornithine
- MS:
-
Murashige and Skoog (1962) medium
- NAA-1:
-
naphthaleneacetic acid
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Kodja, H., Robene-Soustrade, I. & Figier, J. Synergistic effect of DFMO and 2,4-D on regeneration of Tabernaemontana persicariaefolia jacq in vitro . Acta Physiol Plant 19, 359–366 (1997). https://doi.org/10.1007/s11738-997-0013-x
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DOI: https://doi.org/10.1007/s11738-997-0013-x