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A Comparison of the Effectiveness of Sonication, High Shear Mixing and Homogenisation on Improving the Heat Stability of Whey Protein Solutions

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Abstract

Upon ultrasonic treatment at 20 kHz, protein aggregates in a dairy whey solution were broken down. In addition, when sonication was applied to a heated solution of denatured and aggregated proteins, there was a dramatic reduction in viscosity and aggregate size, which was maintained after re-heating. This observed heat stability may be due to shear forces that are induced by acoustic cavitation. To determine whether high shear mixing or homogenisation is able to cause similar effects to that of acoustic cavitation, sonication, high shear mixing and homogenisation were performed on 5 wt% whey protein concentrate solutions at identical energy density levels, which was based on the power drawn in each system. Homogenisation provided similar particle size and viscosity reductions as sonication while high shear mixing was less efficient in decreasing particle size. Cavitation was shown to be absent in both the mixing and homogenisation configurations, indicating that the shear forces generated are responsible for the observed particle size and viscosity reduction. In addition, heat stability was achieved in all systems indicating that a combination of heat treatment and any method that generates high shear forces can be used to improve the heat stability of whey proteins.

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References

  • Alegria, A. E., Lion, Y., Kondo, T., & Riesz, P. (1989). Sonolysis of aqueous surfactant solutions: probing the interfacial region of cavitation bubbles by spin trapping. The Journal of Physical Chemistry, 93(12), 4908–4913.

    Article  CAS  Google Scholar 

  • Ashokkumar, M., Lee, J., Zisu, B., Bhaskarcharya, R., Palmer, M., & Kentish, S. (2009). Sonication increases the heat stability of whey proteins. Journal of Dairy Science, 92(11), 5353–5356.

    Article  CAS  Google Scholar 

  • Bouaouina, H., Desrumaux, A., Loisel, C., & Legrand, J. (2006). Functional properties of whey proteins as affected by dynamic high-pressure treatment. International Dairy Journal, 16(4), 275–284.

    Article  CAS  Google Scholar 

  • Chandrapala, J., Zisu, B., Palmer, M., Kentish, S., & Ashokkumar, M. (2011). Effects of ultrasound on the thermal and structural characteristics of proteins in reconstituted whey protein concentrate. Ultrasonics Sonochemistry, 18(5), 951–957.

    Article  CAS  Google Scholar 

  • Considine, T., Patel, H. A., Anema, S. G., Singh, H., & Creamer, L. K. (2007). Interactions of milk proteins during heat and high hydrostatic pressure treatments—a review. Innovative Food Science and Emerging Technologies, 8(1), 1–23.

    Article  CAS  Google Scholar 

  • Dalgleish, D. G., Senaratne, V., & Francois, S. (1997). Interactions between α-lactalbumin and β-lactoglobulin in the early stages of heat denaturation. Journal of Agricultural and Food Chemistry, 45(9), 3459–3464.

    Article  CAS  Google Scholar 

  • Deysher, E. F., Webb, B. H., & Holm, G. E. (1929). The relations of temperature and time of forewarming of milk to the heat stability of its evaporated product. Journal of Dairy Science, 7(1), 80–89.

    Article  Google Scholar 

  • Dissanayake, M., & Vasiljevic, T. (2009). Functional properties of whey proteins affected by heat treatment and hydrodynamic high-pressure shearing. Journal of Dairy Science, 92(4), 1387–1397.

    Article  CAS  Google Scholar 

  • Floury, J., Bellettre, J., Legrand, J., & Desrumaux, A. (2004). Analysis of a new type of high pressure homogeniser. A study of the flow pattern. Chemical Engineering Science, 59(4), 843–853.

    Article  CAS  Google Scholar 

  • Grácia-Juliá, A., René, M., Cortés-Muñoz, M., Picart, L., López-Pedemonte, T., Chevalier, D., et al. (2008). Effect of dynamic high pressure on whey protein aggregation: a comparison with the effect of continuous short-time thermal treatments. Food Hydrocolloids, 22(6), 1014–1032.

    Article  Google Scholar 

  • Gülseren, İ., Güzey, D., Bruce, B. D., & Weiss, J. (2007). Structural and functional changes in ultrasonicated bovine serum albumin solutions. Ultrasonics Sonochemistry, 14(2), 173–183.

    Article  Google Scholar 

  • Havea, P., Singh, H., Creamer, L. K., & Campanella, O. H. (1998). Electrophoretic characterization of the protein products formed during heat treatment of whey protein concentrate solutions. The Journal of Dairy Research, 65(01), 79–91.

    Article  CAS  Google Scholar 

  • Ika. (2009). Ika Ultra-Turrax T25 Digital Instruction Manual. IKA Works GmbH & Co.

  • Iordache, M., & Jelen, P. (2003). High pressure microfluidization treatment of heat denatured whey proteins for improved functionality. Innovative Food Science and Emerging Technologies, 4(4), 367–376.

    Article  CAS  Google Scholar 

  • Kato, A., Osako, Y., Matsudomi, N., & Kobayashi, K. (1983). Changes in the emulsifying and foaming properties of proteins during heat denaturation. Agricultural and Biological Chemistry, 47(1), 33–37.

    Article  CAS  Google Scholar 

  • Krešić, G., Lelas, V., Jambrak, A. R., Herceg, Z., & Brnčić, S. R. (2008). Influence of novel food processing technologies on the rheological and thermophysical properties of whey proteins. Journal of Food Engineering, 87(1), 64–73.

    Article  Google Scholar 

  • Mahamuni, N. N., & Pandit, A. B. (2006). Effect of additives on ultrasonic degradation of phenol. Ultrasonics Sonochemistry, 13(2), 165–174.

    Article  CAS  Google Scholar 

  • Matsudomi, N., Oshita, T., Kobayashi, K., & Kinsella, J. E. (1993). α-Lactalbumin enhances the gelation properties of bovine serum albumin. Journal of Agricultural and Food Chemistry, 41(7), 1053–1057.

    Article  CAS  Google Scholar 

  • Mleko, S. (2002). Gelation of shear treated whey protein polymers/aggregates. Journal of Food Science and Technology, 39(2), 167–169.

    CAS  Google Scholar 

  • Morr, C. V., & Richter, R. L. (1999). Chemistry of Processing. In N. P. Wong, R. Jenness, M. Keeney, & E. H. Marth (Eds.), Fundamentals of dairy chemistry (pp. 739–766). New York: Aspen.

    Google Scholar 

  • Oldfield, D. J., Singh, H., Taylor, M. W., & Pearce, K. N. (2000). Heat-induced interactions of β-lactoglobulin and α-lactalbumin with the casein micelle in pH-adjusted skim milk. International Dairy Journal, 10(8), 509–518.

    Article  CAS  Google Scholar 

  • Oldfield, D. J., Singh, H., & Taylor, M. W. (2005). Kinetics of heat-induced whey protein denaturation and aggregation in skim milks with adjusted whey protein concentration. The Journal of Dairy Research, 72(03), 369–378.

    Article  CAS  Google Scholar 

  • Oldrup J. (2006). Application: WO Patent No. 2005-DK760.

  • Onwulata, C. I., Konstance, R. P., & Tomasula, P. M. (2002). Viscous properties of microparticulated dairy proteins and sucrose. Journal of Dairy Science, 85(7), 1677–1683.

    Article  CAS  Google Scholar 

  • Paquin, P. (1999). Technological properties of high pressure homogenizers: the effect of fat globules, milk proteins, and polysaccharides. International Dairy Journal, 9(3–6), 329–335.

    Article  CAS  Google Scholar 

  • Sanchez, C., Pouliot, M., Gauthier, S. F., & Paquin, P. (1997). Thermal aggregation of whey protein isolate containing microparticulated or hydrolyzed whey proteins. Journal of Agricultural and Food Chemistry, 45(7), 2384–2392.

    Article  CAS  Google Scholar 

  • Singh, H. (2004). Heat stability of milk. International Journal of Dairy Technology, 57(2/3), 111–119.

    Article  CAS  Google Scholar 

  • Tang, Q., Munro, P. A., & McCarthy, O. J. (1993). Rheology of whey protein concentrate solutions as a function of concentration, temperature, pH and salt concentration. The Journal of Dairy Research, 60(3), 349–361.

    Article  CAS  Google Scholar 

  • Tolkach, A., & Kulozik, U. (2007). Reaction kinetic pathway of reversible and irreversible thermal denaturation of β-lactoglobulin. Le Lait, 87(4–5), 301–315.

    Article  CAS  Google Scholar 

  • van de Hulst, H. C. (1957). Light scattering by small particles. New York: Wiley.

    Google Scholar 

  • Wang, Q., Tolkach, A., & Kulozik, U. (2006). Quantitative assessment of thermal denaturation of bovine α-lactalbumin via low-intensity ultrasound, HPLC, and DSC. Journal of Agricultural and Food Chemistry, 54(18), 6501–6506.

    Article  CAS  Google Scholar 

  • Webb, B. H., & Bell, R. W. (1942). The effect of high-temperature short-time forewarming of milk upon the heat stability of its evaporated product. Journal of Dairy Science, 25(4), 301–311.

    Article  Google Scholar 

  • Zisu, B., Bhaskaracharya, R., Kentish, S., & Ashokkumar, M. (2010). Ultrasonic processing of dairy systems in large scale reactors. Ultrasonics Sonochemistry, 17(6), 1075–1081.

    Article  CAS  Google Scholar 

  • Zisu, B., Lee, J., Chandrapala, J., Bhaskaracharya, R., Palmer, M., Kentish, S., et al. (2011). Effect of ultrasound on the physical and functional properties of reconstituted whey protein powders. The Journal of Dairy Research, 78(2), 226–232.

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by Dairy Innovation Australia, the Particulate Fluids Processing Centre, a Special Research Centre of the Australian Research Council and by the Australian Research Council Linkage Project (LP LP0991048).

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Correspondence to Sandra E. Kentish.

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Koh, L.L.A., Chandrapala, J., Zisu, B. et al. A Comparison of the Effectiveness of Sonication, High Shear Mixing and Homogenisation on Improving the Heat Stability of Whey Protein Solutions. Food Bioprocess Technol 7, 556–566 (2014). https://doi.org/10.1007/s11947-013-1072-1

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  • DOI: https://doi.org/10.1007/s11947-013-1072-1

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