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Kinetic Analysis and Modeling of Daptomycin Batch Fermentation by Streptomyces roseosporus

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Abstract

In this study, Streptomyces roseosporus was subjected to helium–neon (He–Ne) laser (632.8 nm) irradiation to improve the production ability of extracellular antibiotic daptomycin. Under the optimum irradiation dosage of 18 mW for 22 min, a stable positive mutant strain S. roseosporus LC-54 was obtained. The maximum A21978C (daptomycin is a semisynthetic antimicrobial substance derived from the A21978C complex) yield of this mutant strain was 296 mg/l, which was 146% higher than that of the wild strain. The mutant strain grew more quickly and utilized carbohydrate sources more efficiently than the wild strain. The batch culture kinetics was investigated in a 7 l bioreactor. The logistic equation for growth, the Luedeking–Piret equation for daptomycin production, and Luedeking–Piret-like equations for carbon substrate consumption were established. This model appeared to provide a reasonable description for each parameter during the growth phase and fitted fairly well with the experiment data.

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Acknowledgments

The authors wish to acknowledge the financial support provided by the National Natural Science Foundation of China (no. 20706042), National 973 Project of China (2007CB714302), National 863 Project of China (2007AA02Z200), and Program of Introducing Talents of Discipline to Universities (no. B06006).

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Authors and Affiliations

  1. Department of Bioengineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

    Wenyu Lu, Jinghua Fan, Jianping Wen, Zhendong Xia & Qinggele Caiyin

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  1. Wenyu Lu
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  2. Jinghua Fan
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  3. Jianping Wen
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  4. Zhendong Xia
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  5. Qinggele Caiyin
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Correspondence to Qinggele Caiyin.

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Lu, W., Fan, J., Wen, J. et al. Kinetic Analysis and Modeling of Daptomycin Batch Fermentation by Streptomyces roseosporus . Appl Biochem Biotechnol 163, 453–462 (2011). https://doi.org/10.1007/s12010-010-9053-6

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  • DOI: https://doi.org/10.1007/s12010-010-9053-6

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