Abstract
The main objective of this work is to present the optimization of the biotransformation of R-(+)-limonene and (−)-β-pinene aiming at the production of α-terpineol by strains of fungal and yeasts previously isolated by our research group using the methodology of experimental design. New optimized experimental data on α-terpineol production by the biotransformation of R-(+)-limonene and (−)-β-pinene using newly isolated microorganisms are reported in this work. Conversion of about 1,700 mg/L was achieved when R-(+)-limonene was used as substrate and the newly isolated strain 05.01.35 as microorganism at the central point of the experimental design, corresponding to a substrate concentration of 1.75%, mass of inoculum of 2 g, and substrate to ethanol volume ratio of 1:1. The same experimental condition led to higher conversions when (−)-β-pinene was used as substrates and the strains coded as 04.05.08 and 01.04.03 as microorganism. Here, conversions of about 770 mg/L were achieved.
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Rottava, I., Cortina, P.F., Martello, E. et al. Optimization of α-Terpineol Production by the Biotransformation of R-(+)-Limonene and (−)-β-Pinene. Appl Biochem Biotechnol 164, 514–523 (2011). https://doi.org/10.1007/s12010-010-9153-3
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DOI: https://doi.org/10.1007/s12010-010-9153-3