Skip to main content
Log in

Effects of Different Substrate Composition on Biosynthesis of Polyhydroxybutyrate-co-hydroxyvalerate by Recombinant Escherichia coli

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Cupriavidus necator is well known for its ability to accumulate polyhydroxybutyrate (PHB). When supplemented with propionic acid (or sodium propionate) in the growth medium, the bacterium is also able to synthesize polyhydroxybutyrate-co-hydroxyvalerate (PHBV). In order to increase the fraction of 3-hydroxyvalerate (3HV) in PHBV, we cloned the propionate permease gene prpP from C. necator and the propionyl-CoA synthase gene prpE from Cupriavidus taiwanensis and transformed into an Escherichia coli containing phaCAB operon of C. necator. The effects on PHBV accumulation in cells co-expressed with phaCAB and prpE or prpP in the media contained mixed carbon sources (glucose and sodium propionate) were evaluated. The HV fraction in PHBV increased when prpE or prpP was overexpressed in the cells. Concentrations of yeast extracts could also affect the fraction of HV. In addition, when glucose was replaced by sodium pyruvate, sodium succinate, or sodium gluconate, only PHB were detected in the recombinant strains.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Lee, S. Y. (1996). Biotechnology and Bioengineering, 49, 1–14.

    Article  CAS  Google Scholar 

  2. Steinbüchel, A., & Valentin, H. E. (1995). FEMS Microbiology Letters, 128, 219–228.

    Article  Google Scholar 

  3. Sudesh, K., Abe, H., & Doi, Y. (2000). Progress in Polymer Science, 25, 1503–1555.

    Article  CAS  Google Scholar 

  4. Guerrero, R., & Berlanga, M. (2007). International Microbiology, 10, 157–168.

    CAS  Google Scholar 

  5. Tian, G., Wu, Q., Sun, S., Noda, I., & Chen, G.-Q. (2002). J. Polym. Sci. Pt. B-Polym. Phys., 40, 649–656.

    Article  CAS  Google Scholar 

  6. Steinbüchel, A., & Schlegel, H. G. (1991). Molecular Microbiology, 5, 535–542.

    Article  Google Scholar 

  7. Liu, X.-W., Wang, H.-H., Chen, J.-Y., Li, X.-T., & Chen, G.-Q. (2009). Biochemical Engineering Journal, 43, 72–77.

    Article  CAS  Google Scholar 

  8. Chung, Y. J., Cha, H. J., Yeo, J. S., & Yoo, Y. J. E. (1997). Journal of Fermentation and Bioengineering, 83, 492–495.

    Article  CAS  Google Scholar 

  9. Lee, Y.-H., Park, J.-S., & Huh, T.-L. (1997). Biotechnology Letters, 19, 771–774.

    Article  CAS  Google Scholar 

  10. Fidler, S., & Dennis, D. (1992). FEMS Microbiology Reviews, 103, 231–235.

    Article  CAS  Google Scholar 

  11. Slater, S., Gallaher, T., & Dennis, D. (1992). Applied and Environmental Microbiology, 58, 1089–1094.

    CAS  Google Scholar 

  12. Choi, J.-I., & Lee, S. Y. (1999). Applied and Environmental Microbiology, 65, 4363–4368.

    CAS  Google Scholar 

  13. Iadevaia, S., & Mantzaris, N. V. (2006). Journal of Biotechnology, 122, 99–121.

    Article  CAS  Google Scholar 

  14. Iadevaia, S., & Mantzaris, N. V. (2007). Journal of Biotechnology, 128, 615–637.

    Article  CAS  Google Scholar 

  15. Chen, Q., Wang, Q., Wei, G., Liang, Q., & Qi, Q. (2011). Applied and Environmental Microbiology, 77, 4886–4893.

    Article  CAS  Google Scholar 

  16. Wong, M. S., Causey, T. B., Mantzaris, N., Bennett, G. N., & San, K.-Y. (2008). Biotechnology and Bioengineering, 99, 919–928.

    Article  CAS  Google Scholar 

  17. Yim, K. S., Lee, S. Y., & Chang, H. N. (1996). Biotechnology and Bioengineering, 49, 495–503.

    Article  CAS  Google Scholar 

  18. Spiekermann, P., Rehm, B. H., Kalscheuer, R., Baumeister, D., & Steinbüchel, A. (1999). Archives of Microbiology, 171, 73–80.

    Article  CAS  Google Scholar 

  19. Dikshit, K. L., & Webster, D. A. (1988). Gene, 70, 377–386.

    Article  CAS  Google Scholar 

  20. Chang, A. C., & Cohen, S. N. (1978). Journal of Bacteriology, 134, 1141–1156.

    CAS  Google Scholar 

  21. Braunegg, G., Sonnleitner, B., & Lafferty, R. M. (1978). European Journal of Applied Microbiology, 6, 29–37.

    Article  CAS  Google Scholar 

  22. Dong, Z., & Sun, X. (2000). Chinese Science Bulletin, 45, 252–255.

    Article  CAS  Google Scholar 

  23. Chien, C. C., & Ho, L. Y. (2008). Letters in Applied Microbiology, 47, 587–593.

    Article  CAS  Google Scholar 

  24. Doi, Y., Tamaki, A., Kunioka, M., & Soga, K. (1988). Applied Microbiology and Biotechnology, 28, 330–334.

    Article  CAS  Google Scholar 

  25. Aldor, I., & Keasling, J. D. (2001). Biotechnology and Bioengineering, 76, 108–114.

    Article  CAS  Google Scholar 

  26. Chien, C.-C., Hong, C.-C., Soo, P.-C., Wei, Y.-H., Chen, S.-Y., Cheng, M.-L., & Sun, Y.-M. (2010). Applied Biochemistry and Biotechnology, 162, 2355–2364.

    Article  CAS  Google Scholar 

  27. Chen, W.-M., Laevens, S., Lee, T.-M., Coenye, T., De Vos, P., Mergeay, M., & Vandamme, P. (2001). International Journal of Systematic and Evolutionary Microbiology, 51, 1729–1735.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was partially supported by grants (contract number NSC100-2221-E-155-002, NSC99-2628-E-155-002 and NSC98-2622-E-155-003-CC2) from National Science Council, Taiwan.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Chih-Ching Chien.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chien, CC., Li, HH., Soo, PC. et al. Effects of Different Substrate Composition on Biosynthesis of Polyhydroxybutyrate-co-hydroxyvalerate by Recombinant Escherichia coli . Appl Biochem Biotechnol 166, 796–804 (2012). https://doi.org/10.1007/s12010-011-9469-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-011-9469-7

Keywords

Navigation