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Tobacco Arabinogalactan Protein NtEPc Can Promote Banana (Musa AAA) Somatic Embryogenesis

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Abstract

Banana is an important tropical fruit worldwide. Parthenocarpy and female sterility made it impossible to improve banana varieties through common hybridization. Genetic transformation for banana improvement is imperative. But the low rate that banana embryogenic callus was induced made the transformation cannot be performed in many laboratories. Finding ways to promote banana somatic embryogenesis is critical for banana genetic transformation. After tobacco arabinogalactan protein gene NtEPc was transformed into Escherichia coli (DE3), the recombinant protein was purified and filter-sterilized. A series of the sterilized protein was added into tissue culture medium. It was found that the number of banana immature male flowers developing embryogenic calli increased significantly in the presence of NtEPc protein compared with the effect of the control medium. Among the treatments, explants cultured on medium containing 10 mg/l of NtEPc protein had the highest chance to develop embryogenic calli. The percentage of lines that developed embryogenic calli on this medium was about 12.5 %. These demonstrated that NtEPc protein can be used to promote banana embryogenesis. This is the first paper that reported that foreign arabinogalactan protein (AGP) could be used to improve banana somatic embryogenesis.

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Acknowledgments

This work was supported by National Science Foundation of China (No. 31371696 and No. 41201309), Fundamental Scientific Research Funds for CATAS-TCGRI (No. 1630032013022) and Science Foundation of Hainan Province (No. 313065).

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Authors and Affiliations

  1. Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou, China

    H. Shu, J. Li, Z. Jin & S. Chang

  2. The key lab of Hainan banana genetics and breeding, Haikou, China

    H. Shu, J. Li, Z. Jin & S. Chang

  3. Department of Biology, Zhengzhou University, Zhengzhou, China

    H. Shu

  4. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, China

    L. Xu, Z. Li & S. Chang

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  1. H. Shu
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  2. L. Xu
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  3. Z. Li
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  4. J. Li
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  5. Z. Jin
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  6. S. Chang
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Correspondence to S. Chang.

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Shu, H., Xu, L., Li, Z. et al. Tobacco Arabinogalactan Protein NtEPc Can Promote Banana (Musa AAA) Somatic Embryogenesis. Appl Biochem Biotechnol 174, 2818–2826 (2014). https://doi.org/10.1007/s12010-014-1228-0

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  • DOI: https://doi.org/10.1007/s12010-014-1228-0

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