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A Preliminary and Qualitative Metallomics Study of Mercury in the Muscle of Fish from Amazonas, Brazil

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Abstract

This paper presents preliminary findings for a metallomics study of mercury in the muscle of the fish species from Amazonas, Brazil, after protein separation by two-dimensional polyacrylamide gel electrophoresis and subsequent evaluation of mercury by synchrotron radiation X-ray fluorescence. The fluorescence spectra revealed mercury in two protein spots. The mercury-containing protein spots showed molecular weights of 20.8 ± 0.7 and 19.8 ± 0.5 kDa and isoelectric points of 5.6 ± 0.2 and 7.5 ± 0.3, respectively.

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Acknowledgments

The authors thank the Brazilian research funding agency FAPESP (Process: 2010/51332-5 and 2009/54856-8) for financial support.

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Authors and Affiliations

  1. Instituto de Biociências, UNESP, Botucatu, SP, Brazil

    Paula M. Moraes, Felipe A. Santos, Cilene C. F. Padilha, José C. S. Vieira & Pedro de M. Padilha

  2. Campus de Planaltina, Universidade de Brasília – UNB, Brasília, DF, Brazil

    Luiz F. Zara

  3. Instituto Nacional de Ciência e Tecnologia de Bioanalítica, UNICAMP, Campinas, SP, Brazil

    Pedro de M. Padilha

Authors
  1. Paula M. Moraes
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  2. Felipe A. Santos
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  3. Cilene C. F. Padilha
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  4. José C. S. Vieira
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  5. Luiz F. Zara
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  6. Pedro de M. Padilha
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Corresponding author

Correspondence to Pedro de M. Padilha.

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Moraes, P.M., Santos, F.A., Padilha, C.C.F. et al. A Preliminary and Qualitative Metallomics Study of Mercury in the Muscle of Fish from Amazonas, Brazil. Biol Trace Elem Res 150, 195–199 (2012). https://doi.org/10.1007/s12011-012-9502-x

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  • DOI: https://doi.org/10.1007/s12011-012-9502-x

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