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Improvement of Heart Redox States Contributes to the Beneficial Effects of Selenium Against Penconazole-Induced Cardiotoxicity in Adult Rats

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Abstract

The present study was performed to evaluate the protective effect of selenium (Se) against penconazole (PEN)-induced oxidative stress in the cardiac tissue of adult rats. Male Wistar rats were divided into four groups of six each. The first group represented the controls. For the second group (PEN), no treatment was performed during the first 6 days, and then, the rats received intraperitoneally 67 mg/kg body weight (bw) of PEN every 2 days from day 7 until day 15, the sacrifice day. For the third group (Se + PEN), Se was administered daily through the diet at a dose of 0.5 mg/kg of diet for 15 days. Rats of this group received also every 2 days PEN (67 mg/kg bw) from day 7 until day 15. The fourth group (Se) received daily, through the diet, Se (0.5 mg/Kg of diet) during 15 days. Our results showed that Se reduced significantly the elevated cardiac levels of malondialdehyde and protein carbonyl following PEN treatment, and attenuated DNA fragmentation induced by this fungicide. In addition, Se modulated the alterations of antioxidant status: enzymatic (superoxide dismutase, glutathione peroxidase, and catalase) and nonenzymatic (glutathione and vitamin C) antioxidants in the heart of PEN-treated rats. This trace element was also able to alleviate perturbations of lipid profile. The protective effect of selenium was further evident through the histopathological changes produced by PEN in the heart tissue. Taken together, our results indicated that Se might be beneficial against PEN-induced cardiac oxidative damage in rats.

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Abbreviations

AChE:

Acetylcholinesterase

AI:

Atherogenic index

Bw:

Body weight

CAT:

Catalase

DTNB:

5,5′-dithiobis-2-nitrobenzoic acid

GPx:

Glutathione peroxidase

GSH:

Reduced glutathione

HDL-C:

High-density lipoprotein cholesterol

LDL-C:

Low-density lipoprotein cholesterol

MDA:

Malondialdehyde

MRL:

Maximum residue limits

Na2SeO3 :

Sodium selenite

PCO:

Protein carbonyl

PEN:

Penconazole

ROS:

Reactive oxygen species

Se:

Selenium

SOD:

Superoxide dismutase

TBA:

Thiobarbituric acid

TC:

Total cholesterol

TG:

Triglyceride

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Acknowledgments

This work was supported by the Ministry of Higher Education and Scientific Research [UR/11ES-70], Tunisia. The authors are thankful to Mr. Chedli Tmar for the maintenance of the laboratory animals and to Mrs. Raoudha Ben Amar Abdennadher for her skillful technical assistance.

Conflict of Interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Enzymes and Bioconversion Unit, National Engineering School of Sfax, University of Sfax, 3038, Sfax, Tunisia

    Mariem Chaâbane & Raoudha Ellouze Ghorbel

  2. Animal Physiology Laboratory, Department of Life Sciences, Sfax Faculty of Sciences, University of Sfax, BP 1171, 3000, Sfax, Tunisia

    Mariem Chaâbane, Najiba Zeghal & Nejla Soudani

  3. Physiology and aquatic environment laboratory, UR13 ES 35, Tunis Faculty of Sciences, 2092, University of Tunis El Manar, Farhat Hached University Campus El Manar, Tunis. B.P.n 94, 1068, Rommana, Tunisia

    Meriem Tir

  4. Biochemistry Laboratory, University of Sfax, CHU Hedi Chaker, 3029, Sfax, Tunisia

    Safa Hamdi & Kamel Jamoussi

  5. Histopathology Laboratory, University of Sfax, CHU Habib Bourguiba, 3029, Sfax, Tunisia

    Ons Boudawara & Tahia Boudawara

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  1. Mariem Chaâbane
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  2. Meriem Tir
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Correspondence to Najiba Zeghal.

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Chaâbane, M., Tir, M., Hamdi, S. et al. Improvement of Heart Redox States Contributes to the Beneficial Effects of Selenium Against Penconazole-Induced Cardiotoxicity in Adult Rats. Biol Trace Elem Res 169, 261–270 (2016). https://doi.org/10.1007/s12011-015-0426-0

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