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Genotoxicity Test of Self-Renovated Ceramics in Primary Human Peripheral Lymphocytes

  • Translational Biomedical Research
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Abstract

Zirconia-based ceramics is widely used in dentistry. Different compositions of ceramics have different features. Our self-renovated ceramics become more machinable without scarifying its dental restoration properties after adjusting ratio of lanthanum phosphate (LaPO4)/yttrium oxide (Y2O3). In order to evaluate its safety, here, we tested its genotoxicity in primary human peripheral lymphocytes. The human lymphocytes cultured on three groups of different ratios of LaPO4/Y2O3 diphase ceramics for 6 days showed little effect of growth inhibition and similar effect of growth trend to the negative control. Furthermore, single-cell gel electrophoresis (comet assay) indicated that there was no significant difference of the value of tail moment between the tested ceramics and negative control, the IPS Empress II (P > 0.05). Our findings implicate that our self-renovated ceramics do not induce DNA damages in human peripheral lymphocytes and support their future clinic application.

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Authors and Affiliations

  1. Department of Stomatology, Shanghai Yangsi Hospital, Shanghai, 200126, People’s Republic of China

    Nan Hua & Jing Zhuang

  2. Department of Stomatology, Xin Hua Hospital, Shanghai Jiaotong University, Shanghai, 200092, People’s Republic of China

    Huifang Zhu

  3. Department of Prosthodontics, Ninth Peoples Hospital, School of Stomatology, Shanghai Jiaotong University, Shanghai, 200011, People’s Republic of China

    Liping Chen

Authors
  1. Nan Hua
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  2. Huifang Zhu
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  3. Jing Zhuang
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  4. Liping Chen
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Correspondence to Liping Chen.

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Hua, N., Zhu, H., Zhuang, J. et al. Genotoxicity Test of Self-Renovated Ceramics in Primary Human Peripheral Lymphocytes. Cell Biochem Biophys 70, 1773–1777 (2014). https://doi.org/10.1007/s12013-014-0127-x

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  • DOI: https://doi.org/10.1007/s12013-014-0127-x

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