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Statistical Optimization of Medium Components for Mass Production of Plant Growth-Promoting Microbial Inoculant Pseudomonas trivialis BIHB 745 (MTCC5336)

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Abstract

Optimizing nutritional requirements for mass production of microbial inoculants in shortened time has relevance for their economical field application. Therefore, the present study aimed at selecting suitable growth medium, optimizing its components, and up-scaling inoculum production for plant growth-promoting Pseudomonas trivialis BIHB 745. Of the different media tested, the culture exhibited maximal viable colony count in trypticase soya broth with 17.6 % increased biomass on optimizing levels of carbon source, nitrogen source, and NaCl using response surface methodology. A twofold higher biomass with 9 h shorter incubation period was obtained in optimized medium in a bioreactor in comparison to shake flasks.

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Acknowledgments

Authors acknowledge the Director, CSIR-Institute of Himalayan Bioresource Technology for providing the necessary laboratory facilities. CSIR, Govt. of India, is also acknowledged for the financial support under the CSIR Network Project “Exploitation of India’s Rich Microbial Diversity” (NWP-0006).

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Authors and Affiliations

  1. Plant Pathology and Microbiology Laboratory, CSIR-Institute of Himalayan Bioresource Technology, P.O. Box No. 6, Palampur, 176 061, Himachal Pradesh, India

    Pratibha Vyas, Praveen Rahi & Arvind Gulati

  2. Microbiology Department, Guru Nanak Dev University, Amritsar, 143 005, Punjab, India

    B. S. Chadha

Authors
  1. Pratibha Vyas
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  2. Praveen Rahi
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  3. B. S. Chadha
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  4. Arvind Gulati
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Corresponding author

Correspondence to Arvind Gulati.

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Vyas, P., Rahi, P., Chadha, B.S. et al. Statistical Optimization of Medium Components for Mass Production of Plant Growth-Promoting Microbial Inoculant Pseudomonas trivialis BIHB 745 (MTCC5336). Indian J Microbiol 54, 239–241 (2014). https://doi.org/10.1007/s12088-013-0425-9

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  • DOI: https://doi.org/10.1007/s12088-013-0425-9

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