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1H, 13C, 15N assignment and secondary structure determination of glutamine amido transferase subunit of gaunosine monophosphate synthetase from Methanocaldococcus jannaschii

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Abstract

Sequence specific resonance assignments have been obtained for 1H, 13C and 15N nuclei of the 21 kDa (188 residues long) glutamine amido transferase subunit of guanosine monophosphate synthetase from Methanocaldococcus jannaschii. From an analysis of 1H and 13Cα, 13Cβ secondary chemical shifts, 3 JHNHα scalar coupling constants and sequential, short and medium range 1H–1H NOEs, it was deduced that the glutamine amido transferase subunit has eleven strands and five helices as the major secondary structural elements in its tertiary structure.

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Acknowledgments

SPS would like to thank DBT and DST for the NMR facilities at the Indian Institute of Science. RA would like to thank the Indian Institute of Science for a doctoral fellowship. HB would like to thank DBT and DST for funding. SK would like to thank CSIR for a research fellowship.

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Authors and Affiliations

  1. 207, Molecular Biophysics Unit, Indian Institute of Science, Bangalore, 560012, Karnataka, India

    Rustam Ali & Siddhartha P. Sarma

  2. Molecular Biology and Genetics Unit, Jawaharlal Nehru Center for Advanced Scientific Research, Jakkur, Bangalore, 560044, Karnataka, India

    Sanjeev Kumar & Hemalatha Balaram

Authors
  1. Rustam Ali
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  2. Sanjeev Kumar
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  3. Hemalatha Balaram
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  4. Siddhartha P. Sarma
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Correspondence to Siddhartha P. Sarma.

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Ali, R., Kumar, S., Balaram, H. et al. 1H, 13C, 15N assignment and secondary structure determination of glutamine amido transferase subunit of gaunosine monophosphate synthetase from Methanocaldococcus jannaschii . Biomol NMR Assign 6, 193–196 (2012). https://doi.org/10.1007/s12104-011-9354-x

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