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1H, 15N and 13C resonance assignments of the C-terminal domain of the P protein of the Nishigahara strain of rabies virus

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Abstract

The C-terminal domain of the P protein of rabies virus is a multifunctional domain that interacts with both viral and host cell proteins. Here we report the 1H, 13C and 15N chemical shift assignments of this domain from P protein of the Nishigahara strain of rabies virus, a pathogenic laboratory strain well established for studies of virulence functions of rabies virus proteins, including P protein. The data and secondary structure analysis are in good agreement with the reported predominantly helical structure of the same domain from the CVS strain of rabies solved by crystallography. These assignments will enable future solution studies of the interactions of the P protein with viral and host proteins, and the effects of post-translational modifications.

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Acknowledgements

This work was supported by National Health and Medical Research Council project Grant 1125704 (to PRG and GWM); and Australian Research Council equipment Grant LE120100022.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University of Melbourne, Parkville, VIC, 3010, Australia

    Jingyu Zhan, Md. Alamgir Hossain, Ashish Sethi, Gregory W. Moseley & Paul R. Gooley

  2. Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, VIC, 3010, Australia

    Jingyu Zhan, Md. Alamgir Hossain, Ashish Sethi, Gregory W. Moseley & Paul R. Gooley

  3. Faculty of Advanced Life Science, Hokkaido University, Sapporo, 060-0810, Japan

    Toyoyuki Ose

  4. Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton Campus, Clayton, VIC, 3800, Australia

    Gregory W. Moseley

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  1. Jingyu Zhan
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  2. Md. Alamgir Hossain
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  3. Ashish Sethi
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  4. Toyoyuki Ose
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  5. Gregory W. Moseley
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  6. Paul R. Gooley
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Correspondence to Paul R. Gooley.

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Zhan, J., Hossain, M.A., Sethi, A. et al. 1H, 15N and 13C resonance assignments of the C-terminal domain of the P protein of the Nishigahara strain of rabies virus. Biomol NMR Assign 13, 5–8 (2019). https://doi.org/10.1007/s12104-018-9841-4

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  • DOI: https://doi.org/10.1007/s12104-018-9841-4

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