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Cellulase On-Site Production from Sugar Cane Bagasse Using Penicillium echinulatum

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Abstract

Penicillium echinulatum was evaluated as a cellulolytic enzyme producer in shaking flasks and bioreactor submerged culture using sugarcane bagasse as carbon source. Sodium hydroxide delignified steam-exploded pretreated bagasse (SDB) and hydrothermal pretreated bagasse had a maximum filter paper activity (FPase) of 2.4 and 2.6 FPU/mL, respectively. Delignified acid pretreated bagasse and Celufloc 200TM (CE) carbon sources displayed maximum FPase of 1.3 and 1.6 FPU/mL while in natura bagasse (INB) provided the lowest enzyme activity, ca. 0.4 FPU/mL. Measurement of surface specific area of lignocellulosic material and scanning electron microscopic images showed a possible correlation between fungal mycelia accessibility to lignocellulosic particles and obtained cellulolytic enzyme activity of fermentation broth. Fed-batch experiments performed in a controlled bioreactor attained the highest value of FPase of 3.7 FPU/mL, enzyme productivity of 25.7 FPU/L h, and enzyme yield from cellulose equal to 134 FPU/g with SDB. Enzyme hydrolysis of steam-pretreated bagasse accomplished with the obtained supernatant of fermentation broth (10 FPU/g of biomass and 5 % w/v) performed better than commercial cellulose complex. The results showed that P. echinulatum has potential to be used as an on-site enzyme platform aiming second bioethanol production from sugarcane lignocellulosic residue.

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Acknowledgments

The authors would like to thank Dr. Aldo J. P. Dillon for providing the P. echinulatum, the technicians of the National Laboratory of Science and Technology of Bioethanol (CTBE) for assistance during the experiments, and LME/LNLS for technical support during the electron microscopy work.

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Authors and Affiliations

  1. Brazilian Bioethanol Science and Technology Laboratory, Rua Giuseppe Maximo Scolfaro 10000, Pólo II de Alta Tecnologia, CP 6192, Campinas, São Paulo, 13083-970, Brazil

    Beatriz Merchel Piovesan Pereira, Thabata Maria Alvarez, Priscila da Silva Delabona, Fabio Márcio Squina & José Geraldo da Cruz Pradella

  2. Biotechnology Institute, University of Caxias do Sul, Rua Francisco Getulio Vargas 1130, Caxias do Sul, Rio Grande do Sul, 95010-550, Brazil

    Aldo José Pinheiro Dillon

Authors
  1. Beatriz Merchel Piovesan Pereira
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  2. Thabata Maria Alvarez
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  3. Priscila da Silva Delabona
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  4. Aldo José Pinheiro Dillon
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  5. Fabio Márcio Squina
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  6. José Geraldo da Cruz Pradella
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Correspondence to José Geraldo da Cruz Pradella.

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Pereira, B.M.P., Alvarez, T.M., da Silva Delabona, P. et al. Cellulase On-Site Production from Sugar Cane Bagasse Using Penicillium echinulatum . Bioenerg. Res. 6, 1052–1062 (2013). https://doi.org/10.1007/s12155-013-9340-5

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  • DOI: https://doi.org/10.1007/s12155-013-9340-5

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