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Sulforaphane protects primary cultures of cortical neurons against injury induced by oxygen-glucose deprivation/reoxygenation via antiapoptosis

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Abstract

Objective

To determine whether sulforaphane (SFN) protects neurons against injury caused by oxygenglucose deprivation/reoxygenation (OGD/R) and, if so, to investigate the possible mechanisms.

Methods

Primary cultures of neurons were prepared from the cerebral cortex of 1-day-old Sprague-Dawley rats. On days 5–6 in vitro, the neurons were exposed to OGD for 1 h, followed by reoxygenation for 24 h. Cells were treated with 0, 0.1, 0.2, 0.5, 1, 2.5, or 5 μmol/L SFN, with or without 10 μmol/L LY294002, a PI3K-specific inhibitor, during OGD/R (a total of 25 h). After 24-h reoxygenation, MTT was used to assess viability and injury was assessed by Hoechst 33258/propidium iodide (PI) staining; immunofluorescence staining and Western blot were performed to detect molecular events associated with apoptosis.

Results

The MTT assay showed that 1 μmol/L SFN significantly increased viability, and Hoechst 33258/PI staining showed that the numbers of injured neurons were reduced significantly in the SFN group. Furthermore, immunofluorescence staining and Western blot showed that SFN increased Bcl-2 and decreased cleaved caspase-3 levels. Moreover, LY294002 inhibited the phosphorylated-Akt expression evoked by SFN, decreased Bcl-2 expression and increased cleaved caspase-3 expression.

Conclusion

SFN protects neurons against injury from OGD/R and this effect may be partly associated with an antiapoptosis pathway.

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Authors and Affiliations

  1. Department of Pathology, Chongqing Medical University, Chongqing, 400016, China

    Xuemei Wu, Shanshan Yu, Yanlin Chen, Jingxian Wu & Yong Zhao

  2. Department of Pathophysiology, Chongqing Medical University, Chongqing, 400016, China

    Jing Zhao

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  1. Xuemei Wu
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  2. Jing Zhao
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  3. Shanshan Yu
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  4. Yanlin Chen
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  5. Jingxian Wu
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  6. Yong Zhao
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Correspondence to Jing Zhao or Yong Zhao.

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Wu, X., Zhao, J., Yu, S. et al. Sulforaphane protects primary cultures of cortical neurons against injury induced by oxygen-glucose deprivation/reoxygenation via antiapoptosis. Neurosci. Bull. 28, 509–516 (2012). https://doi.org/10.1007/s12264-012-1273-z

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  • DOI: https://doi.org/10.1007/s12264-012-1273-z

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