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Bromopropane compounds inhibit osteogenesis by ERK-dependent Runx2 inhibition in C2C12 cells

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Abstract

Bromopropane (BP) is a halogenated alkan compound used in various industries as chemical intermediates, extraction solvents, and degreasing compounds. Halogenated alkan compounds can damage the nervous system, immune system, and hematopoietic and reproductive functions in animals and humans. However, the effect of BPs on bone formation has not yet been examined. This study examined the effects of BPs on osteoblast differentiation and analyzed the mechanisms involved in C2C12, mesenchymal stem cells. BPs dose dependently reduced the alkaline phosphatase activity, expression levels and promoter activity of bone marker genes. Additionally, 1,2-dibromopropane (1,2-DBP) significantly reduced the levels and transcriptional activity of Runx2 and Osterix, major bone transcription factors, in BMP2 induced C2C12 cells. Furthermore, extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) were significantly inhibited by 1,2-DBP. These results demonstrate that BPs inhibit osteoblast differentiation by suppressing Runx2 and Osterix through the ERK/JNK pathway.

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Acknowledgments

This work was supported by a Grant (2010-0026220) from the National Research Foundation of Korea to K.Y. Lee.

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Authors and Affiliations

  1. College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Gwangju, South Korea

    Hyung Min Jeong, You Hee Choi & Kwang Youl Lee

  2. Department of Toxicology, College of Pharmacy, Chungnam National University, Daejeon, South Korea

    Hye Gwang Jeong

  3. College of Pharmacy, Yeungnam University, Gyeongsan, 712-749, South Korea

    Tae Cheon Jeong

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  1. Hyung Min Jeong
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Correspondence to Kwang Youl Lee.

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Jeong, H.M., Choi, Y.H., Jeong, H.G. et al. Bromopropane compounds inhibit osteogenesis by ERK-dependent Runx2 inhibition in C2C12 cells. Arch. Pharm. Res. 37, 276–283 (2014). https://doi.org/10.1007/s12272-013-0178-3

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  • DOI: https://doi.org/10.1007/s12272-013-0178-3

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