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Determination of five active compounds in Artemisia princeps and A. capillaris based on UPLC-DAD and discrimination of two species with multivariate analysis

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Abstract

Five active compounds, chlorogenic acid, 3,5-di-O-caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid, jaceosidin, and eupatilin, in Artemisia princeps (Compositae) were simultaneously determined by ultra-performance liquid chromatography connected to diode array detector. The morphological resemblance between A. princeps and A. capillaris makes it difficult to properly identify species properly. It occasionally leads to misuse or misapplication in Korean traditional medicine. In the study, the discrimination between A. princeps and A. capillaris was optimally performed by the developed validation method, which resulted in definitely a difference between two species. Also, it was developed the most reliable markers contributing to the discrimination of two species by the multivariate analysis methods, such as a principal component analysis and a partial least squares discrimination analysis.

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Acknowledgments

This work was supported by the Global Leading Technology Program of the Office of Strategic R&D Planning (OSP grant 10039303) funded by the Ministry of Knowledge Economy, Republic of Korea.

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Correspondence to Sang Hyun Sung.

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Yang, H., Lee, D.Y., Jeon, M. et al. Determination of five active compounds in Artemisia princeps and A. capillaris based on UPLC-DAD and discrimination of two species with multivariate analysis. Arch. Pharm. Res. 37, 617–625 (2014). https://doi.org/10.1007/s12272-013-0204-5

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