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Simultaneous analysis and peroxynitrite-scavenging activity of galloylated flavonoid glycosides and ellagic acid in Euphorbia supina

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Abstract

The herbs of Euphorbia supina (Euphorbiaceae) have been used to treat hemorrhage, chronic bronchitis, hepatitis, jaundice, diarrhea, gastritis, and hemorrhoids as a medicinal herb. This work is aimed to qualitatively and quantitatively analyze the polyphenols with peroxynitrite-scavenging activities. The eight compounds: gallic acid, methyl gallate, avicularin, astragalin, juglanin, isoquercitrin 6″-gallate, astragalin 6″-gallate, and ellagic acid, were isolated from E. supina and used for HPLC analysis and peroxynitrite (ONOO−)-scavenging assay. Simultaneous analysis of the eight compounds was performed on MeOH extract and its fractions. The contents in MeOH extract and peroxynitrite-scavenging activities of the dimer of gallic acid, ellagic acid (15.64 mg/g; IC50 0.89 μM), and two galloylated flavonoid glycosides, astragalin 6″-gallate (13.72 mg/g; IC50 1.43 μM) and isoquercitrin 6″-gallate (16.99 mg/g; IC50 1.75 μM), were high, compared to other compounds. The legendary uses of E. supina could be attributed to the high content of polyphenols, particularly ellagic acid, isoquercitrin 6″-gallate, and astragalin 6″-gallate as active principles.

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Acknowledgments

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (# 20100021039).

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Authors and Affiliations

  1. Department of Applied Plant Sciences, Graduate School, Sangji University, Wonju, 220-702, Korea

    Agung Nugroho

  2. Department of Agro-industrial Technology, Faculty of Agriculture, Lambung Mangkurat University, Banjarbaru, 70712, Indonesia

    Agung Nugroho

  3. Department of Animal Biotechnology, Sangji University, Wonju, 220-702, Korea

    Tae-Jin Rhim

  4. Department of Food Science and Nutrition, Sangji University, Wonju, 220-702, Korea

    Moo-Young Choi

  5. Department of Food Science and Nutrition, Pukyong National University, Busan, 607-737, Korea

    Jae Sue Choi

  6. College of Pharmacy, Wonkwang University, Iksan, 570-749, Korea

    Youn-Chul Kim

  7. College of Pharmacy, Sunchon National University, Sunchon, 540-950, Korea

    Min-Sun Kim

  8. Department of Pharmaceutical Engineering, Sangji University, Wonju, 220-702, Korea

    Hee-Juhn Park

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  1. Agung Nugroho
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  2. Tae-Jin Rhim
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  3. Moo-Young Choi
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  4. Jae Sue Choi
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  7. Hee-Juhn Park
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Correspondence to Hee-Juhn Park.

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Nugroho, A., Rhim, TJ., Choi, MY. et al. Simultaneous analysis and peroxynitrite-scavenging activity of galloylated flavonoid glycosides and ellagic acid in Euphorbia supina . Arch. Pharm. Res. 37, 890–898 (2014). https://doi.org/10.1007/s12272-013-0307-z

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  • DOI: https://doi.org/10.1007/s12272-013-0307-z

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