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Ferritin surplus in mouse spleen 14 months after intravenous injection of iron oxide nanoparticles at clinical dose

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Abstract

In this study, we followed the biodegradation of ultra-small superparamagnetic iron oxide nanoparticles injected intravenously at clinical doses in mice. An advanced fitting procedure for magnetic susceptibility curves and low-temperature hysteresis loops was used to fully characterize the magnetic size distribution as well as the magnetic anisotropy energy of the injected P904 nanoparticles (Guerbet Laboratory). Additional magnetometry measurements and transmission electronic microscopy observations were systematically performed to examine dehydrated samples from the spleen and liver of healthy C57B16 mice after nanoparticle injection, with sacrifice of the mice for up to 14 months. At 3 months after injection, the magnetic properties of the spleen and liver were dramatically different. While the liver showed no magnetic signals other than those also present in the reference species, the spleen showed an increased magnetic signal attributed to ferritin. This surplus of ferritin remained constant up to 14 months after injection.

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Author information

Author notes

  1. Arnaud Hillion

    Present address: Institut Jean Lamour, UMR 7198 – Université de Lorraine, Parc de Saurupt, F-54011, Nancy, France

Authors and Affiliations

  1. Institut Lumière Matière, UMR5306 Université Lyon 1- CNRS, Université de Lyon, 69622, Villeurbanne cedex, France

    Alexandre Tamion, Matthias Hillenkamp, Arnaud Hillion, Marie-Geneviève Blanchin & Véronique Dupuis

  2. National Institute of Materials Physics, 105bis Atomistilor St., R-077125, Bucharest-Magurele, Romania

    Valentin A. Maraloiu, Ioana D. Vlaicu, Mariana Stefan & Daniela Ghica

  3. CNRS, UMR 5220, INSERM, U1044, INSA de Lyon, Université de Lyon, Lyon 1, CREATIS, 69003, Lyon, France

    Hugo Rositi & Marlène Wiart

  4. CRNL-BIORAN, CNRS UMR5292, INSERM U1028, Université Lyon 1, 59 BD PINEL, 69677, Bron, France

    Fabien Chauveau

Authors
  1. Alexandre Tamion
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  2. Matthias Hillenkamp
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  4. Valentin A. Maraloiu
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  5. Ioana D. Vlaicu
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  8. Hugo Rositi
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  12. Véronique Dupuis
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Correspondence to Alexandre Tamion or Matthias Hillenkamp.

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Tamion, A., Hillenkamp, M., Hillion, A. et al. Ferritin surplus in mouse spleen 14 months after intravenous injection of iron oxide nanoparticles at clinical dose. Nano Res. 9, 2398–2410 (2016). https://doi.org/10.1007/s12274-016-1126-6

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  • DOI: https://doi.org/10.1007/s12274-016-1126-6

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