Abstract
A putative laccase cDNA from a white-rot basidiomycete, Trametes versicolor, that consisted of 1,769 nucleotides was cloned using the rapid amplification of cDNA ends (RACE)-PCR method. The deduced amino acid sequence had 4 putative copper binding regions, which are common to fungal laccases. In addition, the sequence was 57∼97 % homologous to sequences of other T. versicolor laccases. Additionally, the expression of laccase and manganese peroxidase in this fungus were both greatly increased under degrading conditions for bisphenol A, nonylphenol and two phthalic esters (benzylbutylphthalate and diethylphthalate), all of which are reportedly endocrine disrupting chemicals (EDCs). Furthermore, the estrogenic activities of the EDCs also decreased rapidly during incubation when examined in a two-hybrid yeast system. Finally, kojic acid inhibited the removal of estrogenic activities generated by bisphenol A and nonylphenol, which confirmed that laccase was involved in the degradation of EDCs in T. versicolor.
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Berkmans, P., H. Leppens, C. Vangenechten, and H. Witters. 2007. Screening of endocrine disrupting chemicals with MELN cells, an ER-transactivation assay combined with cytotoxicity assessment. Toxicol. In Vitro 21, 1261–1267.
Bumpus, J.A. and M. Tatarko. 1994. Biodegradation of 2,4,6-trinitrotoluene by Phanerochaete chrysosporium: Identification of initial degradation products and the discovery of a TNT metabolite that inhibits lignin peroxidases. Curr. Microbiol. 28, 185–190.
Cabana, H., J.L. Jiwan, R. Rosenberg, V. Elisashvili, M. Penninckx, S. Agathos, and J. Jones. 2007. Elimination of endocrine disrupting chemicals nonylphenol and bisphenol A and personal care product ingredient triclosan using enzyme preparation from the white rot fungus Coriolopsis polyzona. Chemosphere 67, 770–778.
Champagne, P.P. and J.A. Ramsay. 2005. Contribution of manganese peroxidase and laccase to dye decoloration by Trametes versicolor. Appl. Microbiol. Biotechnol. 69, 276–285.
Cheong, S., S. Yeo, H.G. Song, and H.T. Choi. 2006. Determination of laccase gene expression during degradation of 2,4,6-trinitrotoluene and its catabolic intermediates in Trametes versicolor. Microbiol. Res. 161, 316–320.
Colborn, T., D. Dumanoski, and J. Meyers. 1996. Our Stolen Future. Dutton, New York, N.Y., USA
Dhooge, W., K. Arijs, I. D’Haese, S. Stuyvaert, B. Versonnen, C. Janssen, W. Verstaete, and F. Comhaire. 2006. Experimental parameters affecting sensitivity and specificity of a yeast assay for estrogenic compounds: results of an interlaboratory validation exercise. Anal. Bioanal. Chem. 386, 1419–1428.
Fu, K.Y., C.Y. Chen, and W.M. Chang. 2007. Application of a yeast estrogen screen in non-biomarker species Varicorhinus barbatulus fish with two estrogen receptor subtypes to assay xenoestrogens. Toxicol. In Vitro 21, 604–612.
Gavril, M. and P.V. Hodson. 2007. Decolorization of Amaranth by the white rot fungus Trametes versicolor. Part II. Verification study. Can. J. Microbiol. 63, 327–336.
Han, M.J., H.T. Choi, and H.G. Song. 2004. Degradation of phenanthrene by Trametes versicolor and its laccase. J. Microbiol. 42, 94–98.
Hirano, T., Y. Honda, T. Watanabe, and M. Kuwahara. 2000. Degradation of bisphenol A by the lignin-degrading enzyme, manganese peroxidase, produced by the white-rot basidiomycete, Pleurotus ostreatus. Biosci. Biotechnol. Biochem. 64, 1958–1962.
Kim, K., Y. Leem, K. Kim, K. Kim, and H.T. Choi. 2002. Transformation of medicinal basidiomycete Tramets versicolor to hygromycin B resistance by restriction enzyme mediated integration. FEMS Microbiol. Lett. 209, 273–276.
Kim, H. and H.G. Song. 2003. Transformation and mineralization of 2,4,6-trinitrotoluene by the white rot fungus Irpex lacteus. Appl. Microbiol. Biotechnol. 61, 150–156.
Kim, Y., S. Yeo, J. Keum, H.G. Song, and H.T. Choi. 2005. Cloning of a manganese peroxidase cDNA gene repressed by manganese in Trametes versicolor. J. Microbiol. 43, 569–571.
Ko, E., Y. Leem, and H.T. Choi. 2001. Purification and characterization of laccase isozymes from the white-rot basidiomycete Ganoderma lucidum. Appl. Microbiol. Biotechnol. 57, 98–102.
Kollmann, A., F.D. Boyer, P.H. Ducrot, L. Kerhoas, C. Jolivalt, I. Touton, J. Einhorn, and C. Mougin. 2005. Oligomeric compounds formed from 2,5-xylidine (2,5-dimethylaniline) are potent enhancers of laccase production in Trametes versicolor ATCC 32745. Appl. Microbiol. Biotechnol. 68, 251–258.
Lee, S.M., B.W. Koo, S.S. Lee, M.K. Kim, D.H. Choi, E.J. Hong, E.B. Jeung, and I.G. Choi. 2004. Biodegradation of dibutylphthalate by white rot fungi and evaluation on its estrogenic activity. Enzyme Microb. Technol. 16, 226–231.
Lee, S.M., J.W. Lee, B.W. Koo, M.K. Kim, D.H. Choi, and I.G. Choi. 2007. Dibutyl phthalate biodegradadtion by the white rot fungus, Polyporus brumalis. Biotechnol. Bioeng. 97, 1516–1522.
Macro-Urrea, E., T. Parella, X. Gabarrell, G. Caminal. T. Vicent, and R.C. Adinarayana. 2008. Mechanistics of trichloroethylene mineralization by the white-rot fungus Trametes versicolor. Chemosphere 70, 404–410.
Mnif, W., J.M. Pascussi, A. Pillon, A. Escande, A. Bartegi, J.C. Nicolas, V. Cavailles, M.J. Duchesne, and P. Balaguer. 2007. Estrogens and antiestrogens activate hpXR. Toxicol. Lett. 170, 19–29.
Mori, T. and R. Kondo. 2002. Oxidation of chlorinated dibenzo-p-dioxin and dibenzofuran by white rot fungus, Phlebia lindtneri. FEMS Microbiol. Lett. 216, 223–227.
Mougin, C., A. Kollmann, and C. Jolivalt. 2002. Enhanced production of laccase in the fungus Trametes versicolor by the addition of xenobiotics. Biotechnol. Lett. 24, 139–142.
Murao, S., Y. Hinode, E. Matsumura, A. Numata, K. Kawai, H. Ohishi, H. Jin, H. Oyama, and T. Shin. 1992. A novel laccase inhibitor, N-hydroxyglycine, produced by Penicillium citrinum YH-31. Biosci. Biotech. Biochem. 56, 987–988.
Oh, B.S., Y.J. Jung, Y.J. Oh, Y.S. Yoo, and J.W. Kang. 2006. Application of ozone, UV and ozone/UV processes to reduce diethyl phthalate and its estrogenic activity. Sci. Total Environ. 367, 681–693.
Oshiman, K., Y. Tsutsumi, T. Nishida, and Y. Matsumura. 2007. Isolation and characterization of a novel bacterium, Sphingomonas bisphenolicum strain AO1, that degrades bisphenol A. Biodegradation 18, 247–255.
Sato, A., T. Watanabe, Y. Watanabe, K. Harazono, and T. Fukatsu. 2002. Screening for basidiomycetous fungi degrading 2,7-dichlorodibenzo-p-dioxin. FEMS Microbiol. Lett. 213, 213–217.
Song, H. 1997. Biodegradation of aromatic hydrocarbons by several white-rot fungi. J. Microbiol. 35, 66–71.
Staples, C., P. Dorn, G. Klecka, S. O’Block, and L. Harris. 1998. A review of the environmental fate, effects, and exposures of bisphenol A. Chemosphere 36, 2149–2173.
Suzuki, K., H. Hirai, H. Murata, and T. Nishida. 2003. Removal of estrogenic activities of 17β-estradiol and ethinylestradiol by ligninolytic enzymes from white rot fungi. Water Res. 37, 1972–1975.
Tsutsumi, Y., T. Haneda, and T. Nishida. 2001. Removal of estrogenic activities of bisphenol A and nonylphenol by oxidative enzymes from lignin-degrading basidiomycetes. Chemosphere 42, 271–276.
Yeo, S., N. Park, H.G. Song, and H.T. Choi. 2007. Generation of a transformant showing higher manganese peroxidase (MnP) activity by overexpression of MnP gene in Trametes versicolor. J. Microbiol. 45, 213–218.
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Kim, Y., Yeo, S., Song, HG. et al. Enhanced expression of laccase during the degradation of endocrine disrupting chemicals in Trametes versicolor . J Microbiol. 46, 402–407 (2008). https://doi.org/10.1007/s12275-007-0236-y
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DOI: https://doi.org/10.1007/s12275-007-0236-y