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Isolation and characterization of microsatellite loci in a Neotropical ungulate, the lowland tapir (Tapirus terrestris)

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Abstract

Eight loci were characterized in lowland tapir (Tapirus terrestris) from GTn enriched genomic libraries. In addition, variation at five previously isolated loci in Baird’s tapir (T. bardii) was characterized in lowland tapir. Number of alleles per locus ranged from four to nine, with an average of 5.35 alleles/locus. Mean expected and observed heterozygosities (0.68 for both) were high, as was the power to distinguish between individuals (multilocus P ID = 7.9 × 10−10). There was no evidence for linkage disequilibrium, however, two loci showed evidence of departure from Hardy–Weinberg equilibrium, and two loci tested significantly for null alleles. The relatively recent divergence among extant tapir species suggests that these loci have high potential for cross-amplification within Tapiridae.

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References

  • Beaumont MA (1999) Detecting population expansion and decline using microsatellites. Genetics 153:2013–2029

    CAS  PubMed  Google Scholar 

  • Brownstein MJ, Carpten JD, Smith JR (1996) Modulation of non-templated nucleotide addition by tag DNA polymerase: primer modifications that facilitate genotyping. Biotechniques 20:1004–1010

    CAS  PubMed  Google Scholar 

  • De Young RW, Honeycutt RL (2005) The molecular toolbox: genetic techniques in wildlife ecology and management. J Wildl Manage 69:1362–1384

    Article  Google Scholar 

  • DeSalle R, Amato G (2004) The expansion of conservation genetics. Nat Rev Genet 5:702–712

    Article  CAS  PubMed  Google Scholar 

  • Hamilton MB, Pincus EL, Di Fiore A, Fleischer RC (1999) Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. Biotechniques 27:500–507

    CAS  PubMed  Google Scholar 

  • Manel S, Schwartz M, Luikart G, Taberlet P (2003) Landscape genetics: combining landscape ecology and population genetics. Trends Ecol Evol 18:189–197

    Article  Google Scholar 

  • Medici EP, Desbiez A, Gonçalves da Silva A, et al. (eds) (2007) Lowland tapir (Tapirus terrestris) conservation workshop: final report. IUCN/SSC Tapir Specialist Group & IUCN/SSC Conservation Breeding Specialist Group, Teodoro Sampaio, SP, Brasil. Available via http://tapirs.org/Downloads/action-plan/action_plan_lowland_tapir_eng.pdf. Accessed 09 April 2009

  • Norman JE, Ashley MV (2000) Phylogenetics of Perissodactyla and tests of the molecular clock. J Mol Evol 50:11–21

    CAS  PubMed  Google Scholar 

  • Norton J, Ashley M (2004) Genetic variability and population structure among wild Baird’s tapirs. Anim Conserv 7:211–220

    Article  Google Scholar 

  • Peakall R, Smouse PE (2006) Genalex 6: genetic analysis in excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295

    Article  Google Scholar 

  • Raymond M, Rousset F (1995) GENEPOP (version-1.2)—population-genetics software for exact tests and ecumenicism. J Hered 86(24):8–249

    Google Scholar 

  • Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225

    Article  Google Scholar 

  • Rozen S, Skaletsky H (1999) PRIMER3 on the WWW for general users and for biologist programmers. In: Misener S, Krawetz SA (eds) Bioinformatics methods and protocols. Humana Press, Totowa, NJ, pp 365–386

    Chapter  Google Scholar 

  • Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234

    Article  CAS  PubMed  Google Scholar 

  • Trifonov VA, Stanyon R, Nesterenko AI et al (2008) Multidirectional cross-species painting illuminates the history of karyotypic evolution in Perissodactyla. Chromosome Res 16:89–107

    Article  CAS  PubMed  Google Scholar 

  • Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538

    Article  Google Scholar 

  • Williams BW, Etter DR, Linden DW et al (2009) Noninvasive hair sampling and genetic tagging of co-distributed fishers and American martens. J Wildl Manage 73:26–34

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We would like to thank the Argentinean Ex Situ Tapir group and Viviana Quse for supplying the lowland tapir samples used in this study. This study was made possible by a grant from the Copenhagen Zoo (AGS) and a Martha Piper Research award (MAR).

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Authors and Affiliations

  1. Department of Biology, University of British Columbia Okanagan, 3333 University Way, Kelowna, BC, V1V 1V7, Canada

    Anders Gonçalves da Silva, Danielle R. Lalonde & Michael A. Russello

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  1. Anders Gonçalves da Silva
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  2. Danielle R. Lalonde
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  3. Michael A. Russello
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Correspondence to Anders Gonçalves da Silva.

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Gonçalves da Silva, A., Lalonde, D.R. & Russello, M.A. Isolation and characterization of microsatellite loci in a Neotropical ungulate, the lowland tapir (Tapirus terrestris). Conservation Genet Resour 1, 39–41 (2009). https://doi.org/10.1007/s12686-009-9009-8

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  • DOI: https://doi.org/10.1007/s12686-009-9009-8

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