Abstract
A population of a recently discovered Australian endemic plant species in the genus Erythroxylum (Erythroxylaceae), previously thought only to occur on the Cape York Peninsula, was found at Gove in the Northern Territory. As the only population in the Northern Territory it is likely to be listed as critically endangered within the state. Unlike the other populations it was found to produce mainly vegetatively. To investigate levels of clonality in comparison to non-clonal populations and the spatial distribution of genotypes we developed microsatellite markers. Following an enrichment protocol 10 loci were found to be polymorphic after screening twenty plants from a sexually reproducing population. Number of alleles varied from 3 to 11 while expected heterozygosity ranged between 0.14 and 0.84. While these markers will form a valuable part of an ongoing investigation into the conservation of the isolated population, successful cross species amplification indicates their potential use in other studies.
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Acknowledgements
We acknowledge Chris Spain, J.O. Westaway and John Clarkson for collecting leaf material, Rohan Mellick for DNA extractions and Carolyn Porter for advice during the enrichment procedure. This work forms part of ongoing research and investigation into the Gove population by Rio Tinto Alcan Gove.
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An erratum to this article is available at http://dx.doi.org/10.1007/s12686-011-9384-9.
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van der Merwe, M., Christina, J. & Rossetto, M. Microsatellite loci for a new Australian endemic plant species Erythroxylum sp. ‘Cholmondely Creek’ (J.R.Clarkson 9367) (Erythroxylaceae). Conservation Genet Resour 1, 329–331 (2009). https://doi.org/10.1007/s12686-009-9076-x
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DOI: https://doi.org/10.1007/s12686-009-9076-x