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Isolation and characterization of microsatellite loci in two species-at-risk in British Columbia: Great Basin spadefoot (Spea intermontana) and Western painted turtle (Chrysemys picta bellii)

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Abstract

Seventeen polymorphic microsatellite loci were characterized in two Canadian species-at-risk, Great Basin spadefoot (Spea intermontana, N = 9) and the Western painted turtle (Chrysemys picta bellii, N = 8), from GT n enriched genomic libraries and cross-taxa amplification. Number of alleles per locus ranged from 2 to 9, with an average of 3.9 and 4.3 alleles/locus in Great Basin spadefoot and Western painted turtle, respectively. Mean expected heterozygosities were comparatively high (0.43–0.52), as was the power to distinguish between individuals relative to the number of loci characterized per species (multilocus P ID = 9.2 × 10−5–1.6 × 10−5). These loci will be fundamental in generating historical population information in support of conservation efforts for these two imperiled species.

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Acknowledgments

We thank Ingrid Pollet and Christine Braun for assistance in the laboratory. Orville Dyer, Roger Packham, Karl Larsen, and Melissa Tesche supplied tissue samples. Rene McKibbin offered invaluable assistance in obtaining all necessary permits. Environment Canada and the British Columbia Ministry of Environment provided funding for this work.

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Authors and Affiliations

  1. Department of Biology and Centre for Species at Risk and Habitat Studies, University of British Columbia Okanagan, 3333 University Way, Kelowna, BC, V1V 1V7, Canada

    Anders Gonçalves da Silva, Kathleen E. Williams, Stephanie L. Kirk, Karen E. Hodges & Michael A. Russello

  2. Science and Technology Branch, Environment Canada, Delta, BC, Canada

    Christine A. Bishop

Authors
  1. Anders Gonçalves da Silva
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  2. Kathleen E. Williams
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  3. Stephanie L. Kirk
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  4. Christine A. Bishop
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  5. Karen E. Hodges
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  6. Michael A. Russello
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Corresponding author

Correspondence to Michael A. Russello.

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da Silva, A.G., Williams, K.E., Kirk, S.L. et al. Isolation and characterization of microsatellite loci in two species-at-risk in British Columbia: Great Basin spadefoot (Spea intermontana) and Western painted turtle (Chrysemys picta bellii). Conservation Genet Resour 2, 37–40 (2010). https://doi.org/10.1007/s12686-009-9136-2

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  • DOI: https://doi.org/10.1007/s12686-009-9136-2

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