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Development of microsatellite loci in Triglochin procera (Juncaginaceae), a polyploid wetland plant

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Abstract

Eleven polymorphic microsatellite loci were developed from the polyploid wetland plant Triglochin procera (Juncaginaceae). Loci were screened for variability among 20 individuals from each of two populations in Victoria, Australia. The number of alleles amplified per locus ranged from 5 to 17, with a mean of 9.5. Nei’s genetic diversity (H E ) ranged from 0.463 to 0.898 with a mean of 0.725. These primers provide the opportunity to use polymorphic DNA markers to study the population genetic structure, breeding system and dispersal in T. procera and related species.

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Acknowledgments

We thank Kay Morris and Elisa Raulings for sample locations, Mike Gardner for facilitating 454 sequencing and Mark Blacket for advice on using a universal fluorescent label. This project was supported by the Royal Botanic Gardens Melbourne and ARC Linkage grant LP0776494.

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Authors and Affiliations

  1. Royal Botanic Gardens Melbourne, Private Bag 2000, Birdwood Avenue, South Yarra, Melbourne, VIC, 3141, Australia

    E. A. James, G. K. Brown & R. Citroen

  2. School of Botany, The University of Melbourne, Melbourne, VIC, 3010, Australia

    E. A. James & G. K. Brown

  3. Royal Botanic Gardens Sydney, Mrs Macquaries Road, Sydney, NSW, Australia

    M. Rossetto & C. Porter

Authors
  1. E. A. James
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  2. G. K. Brown
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  3. R. Citroen
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  4. M. Rossetto
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  5. C. Porter
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Correspondence to E. A. James.

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James, E.A., Brown, G.K., Citroen, R. et al. Development of microsatellite loci in Triglochin procera (Juncaginaceae), a polyploid wetland plant. Conservation Genet Resour 3, 103–105 (2011). https://doi.org/10.1007/s12686-010-9301-7

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  • DOI: https://doi.org/10.1007/s12686-010-9301-7

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