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Microsatellite loci for the plate-and-pillar coral, Porities rus.

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Abstract

Little is known about species and population-level genetic relatedness for many reef building corals, despite their critical importance as the foundation of coral reef ecosystems. We report the isolation and characterization of seven polymorphic microsatellites from the plate-and-pillar coral, Porites rus. Across all loci, there was a maximum of 18 alleles for the 37 individuals tested. We also tested these primers on 12 other species of Porites. At least one locus amplified in most of the species. These markers should be useful in various genetic applications involving Porities spp.

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References

  • Dieringer D, Schlötterer C (2002) Microsatellite analyzer (MSA)—a platform independent analysis tool for large microsatellite data sets. Mol Ecol Notes 3:167–169

    Article  Google Scholar 

  • Gaither MA, Toonen RJ, Sorenson L, Bowen BW (2009) Isolation and characterization of microsatellite markers for the Crimson Jobfish, Pristipomoides filamentosus (Lutjanidae). Cons Genet Res 2:169–172

    Article  Google Scholar 

  • Garnier-Géré P, Dillmann C (1992) A computer program for testing pairwise linkage disequilibria in subdivided populations. J Hered 83:239

    PubMed  Google Scholar 

  • Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Meth Enzy 395:202–222

    Article  CAS  Google Scholar 

  • Ridgway T, Gates RD (2006) Why are there so few genetic markers available for coral population analyses? Symbiosis 41:1–7

    Article  CAS  Google Scholar 

  • Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. Meth Mol Biol 132:365–386

    CAS  Google Scholar 

  • Toonen RJ (1997) Microsatellites for ecologists: non-radioactive isolation and amplification protocols for microsatellite markers. Available at www2.hawaii.edu/~toonen/files/MsatsV1.pdf. Accessed 11 Jan 2011

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Acknowledgments

B. Bowen, R. Toonen and their students and staff provided advice and laboratory support, P. Jokiel provided P. rus samples, X. Pochon provided pure Zozanthelle DNA, and A Eggers assisted with genotyping. This research and ZHF were supported by a NOAA-NMS Partnership MOA#2005-008/66882 and National Oceanic and Atmospheric Administration (NOAA), National Marine Fisheries Service, Pacific Islands Regional Office; NMFS-PRPO-2010-2001945. This is HIMB contribution #1425.

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Authors and Affiliations

  1. Hawai’i Institute of Marine Biology, University of Hawai’i, Mānoa, P.O. Box 1346, Kāne’ohe, HI, 96744, USA

    Valery Baranets, Zac H. Forsman & Stephen A. Karl

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  1. Valery Baranets
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  2. Zac H. Forsman
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  3. Stephen A. Karl
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Correspondence to Stephen A. Karl.

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Baranets, V., Forsman, Z.H. & Karl, S.A. Microsatellite loci for the plate-and-pillar coral, Porities rus. . Conservation Genet Resour 3, 519–521 (2011). https://doi.org/10.1007/s12686-011-9393-8

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  • DOI: https://doi.org/10.1007/s12686-011-9393-8

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