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Microsatellite marker development for two species of holly-leafed Grevillea and cross-species amplification in the Aspleniifolia/Hookeriana Subgroup (Proteaceae)

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Abstract

We identified 14 informative microsatellite loci from two taxa, Grevillea aquifolium (6) and G. infecunda (8), belonging to the Pteridifolia Group, Aspleniifolia/Hookeriana Subgroup (Proteaceae). Loci were screened for variability among 20 individuals of their source species. The number of alleles observed per locus for G. infecunda ranged from 1 to 3. Subsequent chromosome counts revealed between 20 (normal diploid) and 30 (triploid) chromosomes in different individuals. High levels of variability were observed with the number of alleles per locus ranging from 3 to 17, with a mean of 9.8 across all loci. Nei’s genetic diversity (H E ) ranged from 0.399 to 0.992 with a mean of 0.783. All loci amplified in a further 9–13 taxa from the same subgroup and under the same amplification conditions. These new markers provide useful tools for evolutionary and conservation studies assessing clonality and polyploidy in this important Australian genus.

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Acknowledgments

This project was generously supported by Alcoa Australia and the Royal Botanic Gardens Melbourne.

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Author notes

  1. M. J. Blacket

    Present address: Department of Primary Industries, Knoxfield, VIC, 3180, Australia

Authors and Affiliations

  1. Royal Botanic Gardens Melbourne, Private Bag 2000, Birdwood Avenue, South Yarra, VIC, 3141, Australia

    E. A. James & R. Citroen

  2. School of Botany, The University of Melbourne, Melbourne, VIC, 3010, Australia

    E. A. James & G. K. Brown

  3. Centre for Environmental Stress and Adaptation Research, Department of Genetics, The University of Melbourne, Parkville, VIC, 3010, Australia

    M. J. Blacket

Authors
  1. E. A. James
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  2. G. K. Brown
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  3. R. Citroen
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  4. M. J. Blacket
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Correspondence to E. A. James.

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James, E.A., Brown, G.K., Citroen, R. et al. Microsatellite marker development for two species of holly-leafed Grevillea and cross-species amplification in the Aspleniifolia/Hookeriana Subgroup (Proteaceae). Conservation Genet Resour 4, 137–140 (2012). https://doi.org/10.1007/s12686-011-9493-5

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  • DOI: https://doi.org/10.1007/s12686-011-9493-5

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