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Development of eighteen polymorphic microsatellite loci for the banded coral shrimp, Stenopus hispidus

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Abstract

Eighteen polymorphic microsatellite loci were developed for the banded coral shrimp, Stenopus hispidus, using 454 sequencing of microsatellite enriched genomic libraries. The 18 loci were characterized in 24 individuals from the Indonesian Archipelago. We found a mean of 8.7 alleles per locus and observed levels of heterozygosity ranged from 0.21 to 1.00. Two loci deviated significantly from Hardy–Weinberg equilibrium and no significant evidence of linkage disequilibrium was observed.

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Acknowledgments

We are particularly grateful to the countless helpful Indonesians who, without their kindness and assistance, none of this work would have been possible. We thank the Indonesian government, the Indonesian Institute of Sciences (LIPI) and RISTEK for collection permits, 0085/SIP/FRP/SM/V/2010 and 0133/SIP/FRP/SM/V/2010 under which all collections were made in full accordance with Indonesian laws and regulations. A special thank you is reserved for Mr Onny Nurrahman Marwayana and Ms Hagi Yulia Sugeha. Funding for this work was provided in part by the TOTAL Foundation, University of Hawaii Graduate Student Organization, University of Hawaii Arts and Sciences Student Research Award, University of Hawaii Edmondson Grant, Sigma XI Grants In Aid of Research, The Explorers Club, The Rufford Small Grants Foundation and the University of Hawaii NSF-EPSCoR program (Investing in Multidisciplinary University Activities, EPS-0903833, J. Gaines, PI) in support of the core functional genomics facility at the Hawaii Institute of Marine Biology. This is HIMB contribution #1497 and The School for Ocean and Earth Science and Technology contribution #8681.

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Authors and Affiliations

  1. Hawai‘i Institute of Marine Biology, University of Hawai‘i, Mānoa, P.O. Box 1346, Kāne‘ohe, HI, 96744, USA

    Benjamin J. Wainwright & Stephen A. Karl

  2. Indonesian Institute of Sciences, Research Center for Oceanography, Jl. Pasir Putih I Ancol Timur, Jakarta, 14430, Indonesia

    Irma S. Arlyza

Authors
  1. Benjamin J. Wainwright
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  2. Irma S. Arlyza
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  3. Stephen A. Karl
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Correspondence to Stephen A. Karl.

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Wainwright, B.J., Arlyza, I.S. & Karl, S.A. Development of eighteen polymorphic microsatellite loci for the banded coral shrimp, Stenopus hispidus . Conservation Genet Resour 4, 947–949 (2012). https://doi.org/10.1007/s12686-012-9680-z

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  • DOI: https://doi.org/10.1007/s12686-012-9680-z

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