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Isolation and characterization of thirty microsatellite loci for Atlantic mackerel (Scomber scombrus L.)

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Abstract

Atlantic mackerel (Scomber scombrus L.) is a widely distributed commercially important pelagic species. Little is known about the stock structure of this species, but it is thought to be undergoing a range extension due to environmental changes. Knowledge of the stock structure under these changing conditions is fundamental for effective management. In this paper, 30 highly polymorphic microsatellite loci developed with next generation sequencing are described. The number of alleles per locus ranged from 4 to 39 in two geographically distant populations, observed and expected heterozygosities ranged between 0.370–0.978 and 0.426–0.962, respectively. These loci are an important resource that will allow assessment of the current population genetic structure of this species, and enable monitoring of climate related changes in the species range and distribution.

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References

  • Brownstein MJ, Carpten JD, Smith JR (1996) Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping. Biotechniques 20(6):1004–1006 (1008-1010)

    PubMed  CAS  Google Scholar 

  • Collette BB, Carpenter KE, Polidoro BA, Juan-Jorda MJ, Boustany A, Die DJ, Elfes C, Fox W, Graves J, Harrison LR, McManus R, Minte-Vera CV, Nelson R, Restrepo V, Schratwieser J, Sun CL, Amorim A, Brick Peres M, Canales C, Cardenas G, Chang SK, Chiang WC, de Oliveira Leite N Jr, Harwell H, Lessa R, Fredou FL, Oxenford HA, Serra R, Shao KT, Sumaila R, Wang SP, Watson R, Yanez E (2011) High value and long life-double jeopardy for tunas and billfishes. Science 333(6040):291–292. doi:10.1126/science.1208730

    Article  PubMed  CAS  Google Scholar 

  • Drummond AJ, Ashton B, Buxton S, Cheung M, Cooper A, Duran C, Field M, Heled J, Kearse M, Markowitz S, Moir R, Stones-Havas S, Sturrock S, Thierer T, Wilson A (2011) Geneious v5.5.6. available from http//www.geneious.com

  • Excoffier L, Lischer HE (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10(3):564–567. doi:10.1111/j.1755-0998.2010.02847.x

    Article  PubMed  Google Scholar 

  • ICES (2012) Report of the Working Group on Widely Distributed Stocks (WGWIDE), 21–27 Aug 2012, Lowestoft, United Kingdom. ICES CM 2012/ACOM:15. 931 pp

  • IUCN (2012) IUCN red list of threatened species. Version 2012.2. www.iucnredlist.org. Downloaded on 09 Nov 2012

  • Libungan LA, Olafsdottir G, Skirnisdottir S, Palsson S, Pampoulie C, Bjornsdottir SH, Olafsson K, Oskarsson GJ, Danielsdottir AK (2012) Fourteen new microsatellite markers for Atlantic herring Clupea harengus. J Fish Biol 81(4):1422–1426. doi:10.1111/j.1095-8649.2012.03391.x

    Article  PubMed  CAS  Google Scholar 

  • Raymond M, Rousset F (1995) Genepop (version 1.2) population genetics software for exact tests and ecumenicism. J Hered 86(3):248–249

    Google Scholar 

  • Rice WR (1989) Analyzing tables of statistical tests. Evolution 43(1):223–225. doi:10.2307/2409177

    Article  Google Scholar 

  • Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18(2):233–234

    Article  PubMed  CAS  Google Scholar 

  • Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4(3):535–538. doi:10.1111/j.1471-8286.2004.00684.x

    Article  Google Scholar 

Download references

Acknowledgments

The authors thank Alexandra M. Klonowski, Ágústa Sigfúsdóttir, Steinunn Magnúsdóttir and Snædís Björnsdóttir for laboratory assistance. This research was supported by The Fisheries Research Fund of the Icelandic Ministry of Fisheries and Agriculture and NORA-Nordic Atlantic Cooperation.

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Correspondence to Gudbjorg Olafsdottir.

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Olafsdottir, G., Olafsson, K., Skirnisdottir, S. et al. Isolation and characterization of thirty microsatellite loci for Atlantic mackerel (Scomber scombrus L.). Conservation Genet Resour 5, 491–494 (2013). https://doi.org/10.1007/s12686-012-9835-y

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  • DOI: https://doi.org/10.1007/s12686-012-9835-y

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