Abstract
Atlantic mackerel (Scomber scombrus L.) is a widely distributed commercially important pelagic species. Little is known about the stock structure of this species, but it is thought to be undergoing a range extension due to environmental changes. Knowledge of the stock structure under these changing conditions is fundamental for effective management. In this paper, 30 highly polymorphic microsatellite loci developed with next generation sequencing are described. The number of alleles per locus ranged from 4 to 39 in two geographically distant populations, observed and expected heterozygosities ranged between 0.370–0.978 and 0.426–0.962, respectively. These loci are an important resource that will allow assessment of the current population genetic structure of this species, and enable monitoring of climate related changes in the species range and distribution.
References
Brownstein MJ, Carpten JD, Smith JR (1996) Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping. Biotechniques 20(6):1004–1006 (1008-1010)
Collette BB, Carpenter KE, Polidoro BA, Juan-Jorda MJ, Boustany A, Die DJ, Elfes C, Fox W, Graves J, Harrison LR, McManus R, Minte-Vera CV, Nelson R, Restrepo V, Schratwieser J, Sun CL, Amorim A, Brick Peres M, Canales C, Cardenas G, Chang SK, Chiang WC, de Oliveira Leite N Jr, Harwell H, Lessa R, Fredou FL, Oxenford HA, Serra R, Shao KT, Sumaila R, Wang SP, Watson R, Yanez E (2011) High value and long life-double jeopardy for tunas and billfishes. Science 333(6040):291–292. doi:10.1126/science.1208730
Drummond AJ, Ashton B, Buxton S, Cheung M, Cooper A, Duran C, Field M, Heled J, Kearse M, Markowitz S, Moir R, Stones-Havas S, Sturrock S, Thierer T, Wilson A (2011) Geneious v5.5.6. available from http//www.geneious.com
Excoffier L, Lischer HE (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10(3):564–567. doi:10.1111/j.1755-0998.2010.02847.x
ICES (2012) Report of the Working Group on Widely Distributed Stocks (WGWIDE), 21–27 Aug 2012, Lowestoft, United Kingdom. ICES CM 2012/ACOM:15. 931 pp
IUCN (2012) IUCN red list of threatened species. Version 2012.2. www.iucnredlist.org. Downloaded on 09 Nov 2012
Libungan LA, Olafsdottir G, Skirnisdottir S, Palsson S, Pampoulie C, Bjornsdottir SH, Olafsson K, Oskarsson GJ, Danielsdottir AK (2012) Fourteen new microsatellite markers for Atlantic herring Clupea harengus. J Fish Biol 81(4):1422–1426. doi:10.1111/j.1095-8649.2012.03391.x
Raymond M, Rousset F (1995) Genepop (version 1.2) population genetics software for exact tests and ecumenicism. J Hered 86(3):248–249
Rice WR (1989) Analyzing tables of statistical tests. Evolution 43(1):223–225. doi:10.2307/2409177
Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18(2):233–234
Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4(3):535–538. doi:10.1111/j.1471-8286.2004.00684.x
Acknowledgments
The authors thank Alexandra M. Klonowski, Ágústa Sigfúsdóttir, Steinunn Magnúsdóttir and Snædís Björnsdóttir for laboratory assistance. This research was supported by The Fisheries Research Fund of the Icelandic Ministry of Fisheries and Agriculture and NORA-Nordic Atlantic Cooperation.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Olafsdottir, G., Olafsson, K., Skirnisdottir, S. et al. Isolation and characterization of thirty microsatellite loci for Atlantic mackerel (Scomber scombrus L.). Conservation Genet Resour 5, 491–494 (2013). https://doi.org/10.1007/s12686-012-9835-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-012-9835-y