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Intracerebral Hemorrhage–Induced Brain Injury in Rats: the Role of Extracellular Peroxiredoxin 2

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A Correction to this article was published on 09 August 2019

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Abstract

Red blood cell (RBC) lysis within the hematoma causes brain injury following intracerebral hemorrhage. Peroxiredoxin 2 (PRX-2) is the third most abundant protein in RBCs and this study examined the potential role of PRX-2 in inducing brain injury in rats. First, adult male Sprague-Dawley rats had an intracaudate injection of lysed RBCs or saline. Brains were harvested at 1 h to measure PRX-2 levels. Second, rats had an intracaudate injection of either recombinant PRX-2, heat-inactivated PRX-2, or saline. Third, rats had intracaudate co-injection of lysed RBCs with conoidin A, a PRX-2 inhibitor, or vehicle. For the second and third parts of studies, behavioral tests were performed and all rats had magnetic resonance imaging prior to euthanasia for brain immunohistochemistry and Western blotting. We found that brain PRX-2 levels were increased after lysed RBC injection. Intracaudate injection of PRX-2 resulted in blood-brain barrier disruption, brain swelling, neutrophil infiltration, microglia activation, neuronal death, and neurological deficits. Intracerebral injection of lysed RBCs induced brain injury, which was reduced by conoidin A. These results suggest that extracellular PRX-2 released from hematoma can cause brain injury following brain hemorrhage and could be a potential therapeutic target.

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Change history

  • 09 August 2019

    In Figure 1, molecular weight of peroxiredoxin 2 (PRX-2) is 22 kDa rather than 32 kDa.

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Funding

GX, YH, and RFK were supported by grants NS-090925, NS-096917, NS-106746, and NS-112394 from the National Institutes of Health.

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Correspondence to Ya Hua.

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All institutional and national guidelines for the care and use of laboratory animals were followed.

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Bian, L., Zhang, J., Wang, M. et al. Intracerebral Hemorrhage–Induced Brain Injury in Rats: the Role of Extracellular Peroxiredoxin 2. Transl. Stroke Res. 11, 288–295 (2020). https://doi.org/10.1007/s12975-019-00714-x

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