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GADD45A induces neuropathic pain by activating P53 apoptosis pathway in mice

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Abstract

Background

Neuropathic pain is a common condition with current heights of varying etiology. The therapeutic drugs are also poorly work and often limited by side effects such as dizziness.

Objective

This study aimed to explore the function mechanism of GADD45A in neuropathic pain.

Methods

The DEGs in neuropathic pain mouse model chip were screened by bioinformatics analysis. The expression of GADD45A in SNL model was determined by RT-qPCR and Immunofluorescence assay. The protein expression of p53-apoptosis pathway proteins was determined by western blotting.

Results

Combination analysis of bioinformatics methods revealed that the expression of GADD45A was upregulated in SNL. The results of RT-qPCR assay and Immunofluorescence assay revealed that GADD45A was overexpressed in all of time points SNL model. Furthermore, knockdown of GADD45A in SNL remarkably antagonized the malignance phenotype compared with the Ad-GFP treated SNL. In addition, knockdown of GADD45A downregulated the expression of p53 and reduced the apoptosis of spinal cord nerve cells.

Conclusions

Our study suggests that GADD45A may be a biomarker in the neuropathic pain of mice.

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Authors and Affiliations

  1. Center of Brain, The 3rd Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, 130000, Jilin, China

    Jing Li

  2. Department of Medical Rehabilitation, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 39, Yanhu Avenue, Donghu Ecological Tourism Scenic District, Wuhan, 430077, Hubei, China

    Jia Chen

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  1. Jing Li
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  2. Jia Chen
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Contributions

JL and JC designed the study, supervised the data collection, JL analyzed the data, interpreted the data, JC prepare the manuscript for publication and reviewed the draft of the manuscript. All authors have read and approved the manuscript.

Corresponding author

Correspondence to Jia Chen.

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Conflict of interest

Jing Li and Jia Chen state that there are no conflicts of interest to disclose.

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Ethical approval was obtained from the Ethics Committee of the 3rd Affiliated Hospital of Changchun University of Chinese Medicine.

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13258_2022_1226_MOESM1_ESM.jpg

Figure S GADD45A overexpression induced p53 upregulation. The expression of apoptosis related proteins and p53 in mice model with sham, SNL, SNL+NC, SNL+GADD45A treatment, respectively, analyzed by western blotting. (JPG 724 kb)

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Cite this article

Li, J., Chen, J. GADD45A induces neuropathic pain by activating P53 apoptosis pathway in mice. Genes Genom 44, 1051–1060 (2022). https://doi.org/10.1007/s13258-022-01226-z

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  • DOI: https://doi.org/10.1007/s13258-022-01226-z

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