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Protective effect of sulfated polysaccharides from a Celluclast-assisted extract of Hizikia fusiforme against ultraviolet B-induced photoaging in vitro in human keratinocytes and in vivo in zebrafish

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Abstract

Our previous study evaluated the in vitro and in vivo antioxidant activities of sulfated polysaccharides from a Celluclast-assisted extract of Hizikia fusiforme (HFPS). The results indicate that HFPS possesses potent antioxidant activity and suggest the potential use of HFPS to combat photoaging. In this study, we investigated the ultraviolet (UV) protective effect of HFPS in vitro in keratinocytes (HaCaT cells) and in vivo in zebrafish. The results indicate that HFPS significantly reduced the level of intracellular reactive oxygen species (ROS) and improved the viability of UVB-irradiated HaCaT cells. In addition, HFPS remarkably decreased apoptosis formation in UVB-irradiated HaCaT cells in a dose-dependent manner. The in vivo test results also demonstrate that HFPS significantly reduced intracellular ROS levels, cell death, NO production, and lipid peroxidation levels in UVB-irradiated zebrafish in a dose-dependent manner. These results suggest that HFPS possesses strong in vitro and in vivo UV-protective effects, making it a potential ingredient in the cosmeceutical industry.

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Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2019R1A6A1A03033553).

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Authors and Affiliations

  1. Department of Marine Life Sciences, Jeju National University, Jeju City, Jeju Self-Governing Province, 63243, Republic of Korea

    Lei Wang, Jae Young Oh, Hye-Won Yang, Hyun Soo Kim & You-Jin Jeon

  2. Marine Science Institute, Jeju National University, Jeju City, Jeju Self-Governing Province, 63333, Republic of Korea

    Lei Wang & You-Jin Jeon

Authors
  1. Lei Wang
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  2. Jae Young Oh
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  3. Hye-Won Yang
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  4. Hyun Soo Kim
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  5. You-Jin Jeon
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Contributions

Lei Wang and You-Jin Jeon conceived and designed the experiments; Lei Wang, Jae Young Oh, Hye-Won Yang, Hyun Soo Kim performed experiments and analyzed data; Lei Wang and You-Jin Jeon wrote the paper.

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Correspondence to You-Jin Jeon.

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The authors declare that they have no conflict of interest.

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We declare that all applicable international, national, and/or institutional guidelines for sampling, care, and experimental use of organisms for the study have been followed and all necessary approvals have been obtained.

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Edited by Xin Yu.

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Wang, L., Oh, J.Y., Yang, HW. et al. Protective effect of sulfated polysaccharides from a Celluclast-assisted extract of Hizikia fusiforme against ultraviolet B-induced photoaging in vitro in human keratinocytes and in vivo in zebrafish. Mar Life Sci Technol 1, 104–111 (2019). https://doi.org/10.1007/s42995-019-00006-1

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  • DOI: https://doi.org/10.1007/s42995-019-00006-1

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