Elsevier

Archives of Oral Biology

Volume 39, Issue 8, August 1994, Pages 715-721
Archives of Oral Biology

Hydroxyapatite mineralization and demineralization in the presence of synthetic phosphorylated pentapeptides

https://doi.org/10.1016/0003-9969(94)90099-XGet rights and content

Abstract

A constant composition (CC) method was used to compare the influence of statherin-like N-terminal 5-residue fragments having different amino acids in the terminal position on hydroxyapatite (HAP) growth and dissolution. The CC experiments were done in solutions containing 4.00 × 10−4 mol/l calcium and 2.40 × 10−4 mol/l phosphate. The solutions used in the crystallization studies were super-saturated only with respect to HAP (pH = 7.40, σHAP = 3.60). The CC dissolution studies were done in solutions undersaturated with respect to HAP (pH = 6.00; σHap = −0.39). The HAP mineralization and demineralization processes were markedly inhibited by the negatively charged pentapeptides. Those containing a phosphorylated terminal residue inhibited dissolution to a greater extent than the native statherin fragment having aspartate as the N-terminal residue. Strong dependencies of the degree of inhibition of growth and dissolution reaction rates on the extents of reaction were noted. As the reactions proceeded, the rate inhibition decreased in the case of crystal growth and increased for dissolution.

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