A monoclonal antibody to an interspecies major histocompatibility determinant inhibits a virus-specific T-cell clone

doi:10.1016/0008-8749(82)90103-4

Cellular Immunology

Volume 68, Issue 1, 15 March 1982, Pages 193-198

https://doi.org/10.1016/0008-8749(82)90103-4 Get rights and content

Abstract

The cytotoxicity of an influenza-specific T-cell clone of BALB/c mouse origin for an H-2 compatible, virus-infected target is greatly inhibited by a monoclonal antibody which binds to mouse major histocompatibility complex (MHC) determinants, but was originally prepared against MHC antigens of the Brown Norway (BN) rat. The inhibition is still observed after absorption of the antibody with lymphoid cells from Lewis, but not from BN or Lewis. l N rats. It thus seems that the site blocked by this monoclonal antibody, which interacts with MHC antigens from a number of animal species, is at least close to a determinant on the MHC glycoprotein that is involved in T-cell recognition. This reagent may be useful for comparative analysis of the phylogeny of MHC-restricted T-cell responses in different species.

References (18)

R.M. Zinkernagel et al.
Advan. Immunol
(1979)
G.M. Shearer et al.
Advan. Immunol
(1977)
P.C. Doherty
I.F.C. McKenzie et al.
Immunol. Rev
(1977)
E. Moller
J. Exp. Med
(1965)
J.C. Cerrottini et al.
Advan. Immunol
(1974)
S.J. Burakoff et al.
K.F. Lindahl et al.
Eur. J. Immunol
(1979)
R.V. Blanden et al.
J. Exp. Med
(1979)

There are more references available in the full text version of this article.

Cited by (2)

The development and analysis of species specific and cross reactive monoclonal antibodies to leukocyte differentiation antigens and antigens of the major histocompatibility complex for use in the study of the immune system in cattle and other species
1987, Veterinary Immunology and Immunopathology
We examined the potential of developing a set of species specific and cross reactive monoclonal antibodies (MoAbs) for use in the study of the phylogenetic and functional relation of class I and Class II antigens of the major histocompatibility complex (MHC) and leukocyte differentiation antigens in cattle and other species. Comparing immunization strategies demonstrated the number of hybrids producing cross reactive antibodies can be increased by hyperimmunization of mice with lymphoid cells from multiple species. Comparing various methods of assay (antibody-complement mediated cytotoxicity [CT], enzyme linked immunosorbent assay [ELISA] and flow microfluorimetry [FMF]), revealed FMF is the most useful technique for the primary assay of hybridomas producing MoAbs of potential interest. By using dual parameter and dual fluorescence analysis, we could determine whether a given MoAb reacted with mononuclear cells (lymphocytes and monocytes) and/or granulocytes, and also whether any two MoAbs of different isotype and specificity recognized antigens present on identical or separate populations of leukocytes. Comparing the patterns of MoAb reactivity with leukocytes obtained from cows, goats, sheep, pigs, horses and humans, as well as comparing the patterns of reactivity with a panel of lymphoid cell lines derived from cattle (with enzootic bovine leukemia) and humans (with various forms of leukemia), revealed sets of MoAbs reactive with unique antigenic determinants present on BoLA class I (15 MoAbs) and class II (9 MoAbs) antigens, and also MoAbs reactive with determinants present on leukocyte differentiation antigens (36 MoAbs). Dual fluorescence analysis demonstrated the antigens detected by some MoAbs are predominantly expressed on one lineage of leukocytes while others are expressed on two or more lineages of leukocytes. Dual and single fluorescence analysis also demonstrated the PNA receptor(s) is: 1) expressed on T cells, granulocytes and class II antigen monocytes and 2) absent or expressed in low amount on sIgM⁺ B cells and a newly defined Non T/Non B population of cells. The strategies described for identifying and analyzing the specificity of MoAbs demonstrate the feasibility of developing a set of cross reactive MoAbs for identifying homologous molecules in multiple species and delineating their functional and phylogenetic relation.
Immunological Surveillance of Tumors in the Context of Major Histocompatibility Complex Restriction of T Cell Function
1984, Advances in Cancer Research
The immunological surveillance hypothesis was formulated prior to the realization of the fact that an individual's effector T cells generally see neoantigen if it is appropriately presented in the context of self major histocompatibility complex (MHC) glycoproteins. The biological consequence of this mechanism is that T lymphocytes are focused onto modified cell-surface rather than onto free antigen. The discovery of MHC-restricted T cell recognition and the realization that T cell-mediated immunity is of prime importance in promoting recovery from infectious processes has, thus, changed the whole emphasis of the surveillance argument. T cell surveillance probably operates well in some situations, but is quite ineffective in many others. Part of the reason for this may be that the host response selects tumor clones that are modified so as to be no longer recognized by cytotoxic T cells. The possibility that this reflects changes in MHC phenotype has been investigated and found to be the case for some experimental tumors. The availability of molecular probes for investigating the status of MHC genes in tumor cells—together with the capacity to develop cloned T cell lines, monoclonal antibodies to putative tumor antigens, and cell lines transfected with genes coding for these molecules—indicates the way T cell surveillance may profitably be explored further in both experimental and human situations.

^☆: This work was supported by Grants AI 14162, NS 11036, CA 10815, and CA 15822 from the National Institutes of Health, and by a grant from the National Multiple Sclerosis Society.

²: A research fellow of the French Ministére des Affaires Etrangéres.

³: Present address: Basel Institute for Immunology, 487 Grenzacherstrasse, Basel, Switzerland.

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Short communicationA monoclonal antibody to an interspecies major histocompatibility determinant inhibits a virus-specific T-cell clone☆

Abstract

Advan. Immunol

Advan. Immunol

Immunol. Rev

J. Exp. Med

Advan. Immunol

Eur. J. Immunol

J. Exp. Med

Short communication
A monoclonal antibody to an interspecies major histocompatibility determinant inhibits a virus-specific T-cell clone☆