Neuronal and non-neuronal components in the overflow of labelled adenyl compounds from guinea-pig taenia coli

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Abstract

Guinea-pig taenia coli preparations were pre-incubated in 3H-adenosine and the overflow of 3H-compounds to a superfusing medium was measured during transmural field stimulation in the presence and absence of tetrodoxin. The fractional overflow of 3H-compounds on stimulation of taenia coli pre-incubated in 3H-adenosine at 10−7M was greater than after incubation at 10−5M. About half of the releasable store was blocked by tetrodotoxin. It is proposed that at lower concentrations, there is a selective loading of a releasable store, while at higher concentrations there is a greater loading of non-releasable stores, and that neuronal stores account for about 33% of the 3H-compounds released by transmural stimulation.

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    Present address: Department of Anatomy and Embryology, University College, London, Gower Street, London WC1E 6BT, England.

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