Nutrient requirements of suspension cultures of soybean root cells

doi:10.1016/0014-4827(68)90403-5

Experimental Cell Research

Volume 50, Issue 1, April 1968, Pages 151-158

https://doi.org/10.1016/0014-4827(68)90403-5 Get rights and content

Abstract

The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyacetic acid (2,4-d) has been designed.

The cells required thiamine, 2,4-d and ammonium in addition to the usual mineral salts and sucrose.

Optimum concentrations of nitrate and ammonium were 25 and 2 mM respectively. The highest yield of cells was achieved at an initial pH of 4.5–5.5. During the growth cycle the pH gradually increased to 6.0–6.2.

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¹: Postdoctorate Fellow 1966–1967. Issued as N.R.C. No. 9901.

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Nutrient requirements of suspension cultures of soybean root cells

Abstract

Exptl Cell Res

Biochim. Biophys. Acta

Lloydia

Plant Physiol

Physiol. Plant

Can. J. Biochem